The association between p16 and Ki-67 immunohistostaining and the progression of cervical intraepithelial neoplasia grade 2
The association between p16 and Ki-67 immunohistostaining and the progression of cervical intraepithelial neoplasia grade 2
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DOI：
10.1016/j.ijgo.2015.12.005
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发表时间：
2016-07-01
影响因子：
3.8
通讯作者：
Sekizawa, Akihiko
中科院分区：
文献类型：
--
作者：
Miyamoto, Shingo;Hasegawa, Junichi;Sekizawa, Akihiko
目的：比较p16和Ki-67阳性细胞在宫颈上皮内瘤变2级(CIN2)进展、持续或消退患者中的表达情况。方法：对2004-2011年间在日本一所大学医院接受治疗的经组织学确诊为CIN2的妇女进行了回顾性研究。分析p16和Ki-67的免疫染色模式，并比较疾病进展、持续或消退的患者之间的差异。Kaplan-Meier分析用于评估免疫染色分层的进展率，进展的危险因素的多因素分析采用Cox比例风险模型。结果：分析包括59名进展型女性、35名持续性女性和28名退化型女性。进展期妇女中p16蛋白表达较深(超过一半的宫颈上皮内间隙染色)和超过50%的细胞Ki-67阳性表达明显多于退化期妇女。P16高表达(P<；0.001)和Ki-67比值>50%(P<；0.001)是CIN2进展的危险因素。结论：在CIN2患者中，p16和Ki-67免疫组织化学染色阳性与疾病进展密切相关。(C)2016年国际妇产科联合会。爱思唯尔爱尔兰有限公司出版。版权所有。
Objective: To compare the frequency of p16- and Ki-67-positive cells on immunohistostaining among women with progression, persistence, or regression of cervical intraepithelial neoplasia grade 2 (CIN2). Methods: A retrospective study was conducted of women with CIN2 diagnosed by histology who were treated at a university hospital in Japan during 2004-2011. The immunostaining patterns for p16 and Ki-67 were analyzed and compared between patients with disease progression, persistence, or regression. Kaplan-Meier analysis was used to evaluate the progression rates stratified by immunostaining, and multivariate analysis of risk factors for progression was performed using the Cox proportional hazards model. Results: The analysis included 59 women with progression, 35 women with persistence, and 28 women with regression. Deep p16 expression (staining in more than half of the cervical intraepithelial compartment) and positive Ki-67 staining in more than 50% of cells were significantly more common among women with progression than among those with regression. The risk factors for progression of CIN2 were deep p16 expression (P < 0.001) and a Ki-67 ratio of more than 50% (P < 0.001). Conclusion: Among women with CIN2, positive immunohistostaining for p16 and Ki-67 was strongly associated with disease progression. (C) 2016 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved.