Signal decomposition of transmembrane voltage-sensitive dye fluorescence using a multiresolution wavelet analysis.
Signal decomposition of transmembrane voltage-sensitive dye fluorescence using a multiresolution wavelet analysis.
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DOI：
10.1109/tbme.2011.2143713
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发表时间：
2011-07
期刊：
影响因子：
--
通讯作者：
Kay MW
中科院分区：
文献类型：
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作者：
Asfour H;Swift LM;Sarvazyan N;Doroslovački M;Kay MW
跨膜电压敏感染料的荧光成像用于研究心脏组织中的电激活。然而，荧光信号通常具有低SNR，并且可能受到运动伪影的污染。在这份报告中，我们介绍了一种新的处理方法，荧光跨膜电位（FTMPs）是基于离散小波变换。我们展示了如何fTmp信号可以被分解和重建，形成三个子信号，包含信号噪声（噪声信号），早期去极化阶段的动作电位（rTmp信号），和运动伪影（rMA信号）。一个coiflet4小波用于fTmp分解和重建这些子信号。使用被运动伪影污染的fTmp信号的结果表明，该方法是一个有用的处理步骤，以消除基线漂移，减少噪声，并揭示波前。它简化了fTmps的预处理，以便随后测量激活时间和传导速度。这是一个很有前途的方法，用于研究波前没有侵略性的机械组织约束或机电解偶联剂，是有用的单相机系统，不提供比率成像。
Fluorescence imaging of transmembrane voltage-sensitive dyes is used to study electrical activation in cardiac tissue. However, the fluorescence signals, typically, have low SNRs and may be contaminated with motion artifact. In this report, we introduce a new processing approach for fluoresced transmembrane potentials (fTmps) that is based upon a discrete wavelet transform. We show how fTmp signals can be decomposed and reconstructed to form three subsignals that contain signal noise (noise signal), the early depolarization phase of the action potential (rTmp signal), and motion artifact (rMA signal). A coiflet4 wavelet is used for fTmp decomposition and reconstruction of these subsignals. Results using fTmp signals that are contaminated with motion artifact indicate that the approach is a useful processing step to remove baseline drift, reduce noise, and reveal wavefronts. It streamlines the preprocessing of fTmps for the subsequent measurement of activation times and conduction velocities. It is a promising approach for studying wavefronts without aggressive mechanical tissue constraint or electromechanical uncoupling agents and is, useful for single-camera systems that do not provide for ratiometric imaging.