Mechanisms involved in prostaglandin-induced increase in bone resorption in neonatal mouse calvaria.
Mechanisms involved in prostaglandin-induced increase in bone resorption in neonatal mouse calvaria.
复制标题
前列腺素诱导新生小鼠颅骨骨吸收增加的机制。
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
C. Cottereaux
中科院分区：
文献类型：
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作者：
C. Gardner;R. Blanqué;C. Cottereaux
前列腺素（PG）E1、E2和F2 α在分离的新生儿顶骨培养物中诱导骨吸收，并伴随白细胞介素-6（IL-6）产生的增加。吲哚美辛对PGE 2或相对非选择性EP受体激动剂11-脱氧PGE 1和米索前列醇的反应几乎没有影响，但阻断了PGF 2 α和F受体激动剂氟前列醇的作用，表明通过释放其他前列腺素的间接作用。更有可能的是，在该制剂中，通过刺激F受体介导的野牡丹素产生存在正性自动调节。选择性EP受体激动剂硫前列酮（EP 1，3）和17-苯基三去甲基前列腺素E2（EP 1）的作用表明，EP 2和/或EP 4受体的参与，通过cAMP的信号。米索前列醇对IL-6产生的相对微弱的增加（就再吸收而言）表明这些反应受EP 2和EP 4受体的不同组合控制。PKA激活剂毛喉素在较低浓度（50-500 ng/ml）下诱导骨吸收的小幅增加，但在较高浓度（0.5-5 μ g/ml）下逆转了该效应，并抑制了其他刺激物（PTH、PGE 2）诱导的骨吸收。IL-6的产生仅在较高浓度下显著增加。毛喉素的抑制作用可能是降钙素模拟效应。PMA诱导的吸收和IL-6的产生都被吲哚美辛阻断，表明PKC在前列腺素产生的控制中的作用。
Prostaglandins (PG) E1, E2 and F2alpha induce bone resorption in isolated neonatal parietal bone cultures, and an associated increase in interleukin-6 (IL-6) production. Indomethacin had little effect on the response to PGE2, or the relatively non-selective EP receptor agonists 11-deoxy PGE1 and misoprostol, but blocked the effects of PGF2alpha and the F receptor agonist fluprostenol, indicating an indirect action via release of other prostaglandins. It is more likely that there is positive autoregulation of prostaglandins production in this preparation mediated via stimulation of F receptors. The effects of selective EP receptor agonists sulprostone (EP1,3) and 17-phenyl trinor PGE2(EP1), indicated the involvement of EP2 and/or EP4 receptors, which signal via cAMP. The relatively weak increase in IL-6 production by misoprostol (with respect to resorption) suggests that these responses are controlled by different combination of EP2 and EP4 receptors. The PKA activator, forskolin, induced small increases in bone resorption at lower concentrations (50-500 ng/ml) but a reversal of this effect, and inhibition of resorption induced by other stimuli (PTH, PGE2), at higher concentrations (0.5-5 microg/ml). IL-6 production was markedly increased only at the higher concentrations. The inhibitory effect of forskolin may be a calcitonin-mimetic effect. PMA induced both resorption and IL-6 production which were both blocked by indomethacin, indicating a role for PKC in the control of prostaglandin production.
影响因子：
15.9
作者：
Okada, Y;Lorenzo, JA;Pilbeam, CC
通讯作者：
Pilbeam, CC
小鼠前列腺素 EP2 受体的敲除会损害体外破骨细胞生成。
影响因子：
4.8
作者：
Li,X;Okada,Y;Pilbeam,CC;Lorenzo,JA;Kennedy,CR;Breyer,RM;Raisz,LG
通讯作者：
Raisz,LG