An electrode of quartz crystal microbalance decorated with CNT/chitosan/fibronectin for investigating early adhesion and deforming morphology of rat mesenchymal stem cells
An electrode of quartz crystal microbalance decorated with CNT/chitosan/fibronectin for investigating early adhesion and deforming morphology of rat mesenchymal stem cells
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DOI：
10.1016/j.carbpol.2011.03.025
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发表时间：
2011-07-01
影响因子：
11.2
通讯作者：
Tyan, Yu-Chang
中科院分区：
文献类型：
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作者：
Chung, Tze-Wen;Limpanichpakdee, Thitima;Tyan, Yu-Chang
间充质干细胞（MSCs）对各种生物聚合物的早期反应对其增殖和分化至关重要。在无血清培养条件下，研究了大鼠骨髓间充质干细胞在多壁碳纳米管（CNT）/壳聚糖（CS）、CNT/CS/透明质酸（HA）和CNT/CS/纤连蛋白（FN）修饰的石英晶体微天平（QCM）电极上的粘附情况，并与其他技术进行了比较。细胞计数法测得rMSCs在CNT/CS/FN和CNT电极上的贴壁数分别为初始值的76%和26%，MTS法测得的贴壁数无差异。在QCM的CNT/CS/FN电极上贴壁的rMSCs的重量比其他表面高50%，与细胞计数技术一致。在CNT/CS/FN表面上粘附的rMSCs的形态与其他表面上的明显不同。总之，CNT/CS/FN或CNT/CS/碳水化合物聚合物修饰的QCM电极可以定量测定rMSCs在其表面的早期粘附。(C)2011爱思唯尔有限公司保留所有权利。
The early responses of mesenchymal stem cells (MSCs) to various biopolymers are important to their proliferation and differentiation. Here, the adhesions of rat MSCs on the electrode of quartz crystal microbalance (QCM) decorated with multi-walled carbon nanotubes (CNT)/chitosan (CS), CNT/CS/hyaluronic acid (HA) and CNT/CS/fibronectin (FN) layers were investigated and compared with other techniques at a serum-free condition for 12-h incubation. The numbers of adhered rMSCs on CNT/CS/FN and CNT electrodes were 76 and 26% of the initial values, respectively, determined by cell-count technique while MTS assay could not detect the differences. The weights of adhered rMSCs on CNT/CS/FN electrodes of QCM were 50% higher than those on other surfaces that were consistent with cell-count technique. The morphology of adhered rMSCs on the CNT/CS/FN surfaces markedly differed from those on other surfaces. In conclusion, the electrodes of QCM decorated with CNT/CS/FN or CNT/CS/carbohydrate polymers could be quantified the early adhesion of rMSCs on their surfaces. (C) 2011 Elsevier Ltd. All rights reserved.