Cutting Edge: Identification of Marginal Reticular Cells as Phagocytes of Apoptotic B Cells in Germinal Centers
Cutting Edge: Identification of Marginal Reticular Cells as Phagocytes of Apoptotic B Cells in Germinal Centers
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DOI：
10.4049/jimmunol.1701293
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发表时间：
2018-06-01
影响因子：
4.4
通讯作者：
Shibuya, Kazuko
中科院分区：
文献类型：
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作者：
Sato, Kazuki;Honda, Shin-ichiro;Shibuya, Kazuko
次级淋巴器官中的生发中心（GC）产生大量凋亡的B细胞，这些细胞必须被吞噬细胞清除以防止自身免疫性疾病的发展。虽然易染体巨噬细胞吞噬凋亡的GC B细胞，但基质细胞是否也参与这一过程尚不清楚。在这项研究中，我们确定了边缘网状细胞（MRCs）作为新的非专职吞噬细胞的凋亡GC B细胞在脾脏中的清除。我们使用在CD 19（cre）表达控制下表达增强的GFP（eGFP）的CD 19（eGFP）（CD 19（cre）Z/EG）小鼠，在用NP-鸡γ球蛋白加铝盐免疫后追踪GC中的B细胞。我们证明了MRC群体，如通过podoplanin或Rankl的表达所确定的，在免疫后在细胞质中特异性地显示eGFP信号。这些结果表明，MRCs有助于GC中凋亡B细胞的清除。
Germinal centers (GCs) in secondary lymphoid organs generate large numbers of apoptotic B cells that must be eliminated by phagocytes to prevent the development of autoimmune diseases. Although tingible body macrophages engulf apoptotic GC B cells, whether stromal cells are also involved in this process is unclear. In this study, we identified marginal reticular cells (MRCs) as novel non-professional phagocytes for the clearance of apoptotic GC B cells in the spleen. We used CD19(eGFP) (CD19(cre) Z/EG) mice, which express enhanced GFP (eGFP) under the control of CD19(cre) expression, to track B cells in the GCs after immunization with NP-chicken gamma globulin plus aluminum salt. We demonstrated that the MRC population, as determined by expression of podoplanin or Rankl, specifically showed an eGFP signal in the cytoplasm after immunization. These results suggest that MRCs contribute to the clearance of apoptotic B cells in GCs.