Antimicrobial activity of intraurethrally administered probiotic Lactobacillus casei in a murine model of Escherichia coli urinary tract infection
Antimicrobial activity of intraurethrally administered probiotic Lactobacillus casei in a murine model of Escherichia coli urinary tract infection
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DOI：
10.1128/aac.45.6.1751-1760.2001
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发表时间：
2001-06-01
影响因子：
4.9
通讯作者：
Yokokura, T
中科院分区：
文献类型：
--
作者：
Asahara, T;Nomoto, K;Yokokura, T
在小鼠尿路感染（UTI）模型中，研究了经尿道给药益生菌干酪乳杆菌Shirota菌株对大肠杆菌的抑菌活性。将大肠杆菌HU-1（临床分离自UTI患者，1型和P型菌毛呈阳性）以1 × 10(6)至2 × 10(6) CFU的剂量（20毫升生理盐水）经尿道注入创伤的C3H/HeN小鼠膀胱，在攻毒感染前24小时用0.1 N HCl立即中和0.1 N NaOH，从而诱导UTI。尿路（膀胱和肾脏）慢性感染106 CFU的病原菌，感染后持续3周以上，感染期间尿中多形核白细胞数量和髓过氧化物酶活性明显升高。一个单一的管理。casei Shirota在攻击感染前24小时给予10(8)CFU剂量，显著抑制大肠杆菌生长和尿路炎症反应。感染后每日多次处理干酪乳杆菌（L. casei Shirota）也显示出抗菌活性。热杀干酪乳杆菌（L. casei Shirota）制剂不仅在单次预处理（100杯/只）下具有显著的抗菌效果，而且在感染后每日多次处理也具有显著的抗菌效果。其余乳杆菌菌株发酵乳杆菌ATCC 14931(T)、简氏乳杆菌ATCC 25258(T)、植物乳杆菌ATCC 14917(T)和罗伊氏乳杆菌JCM 1112(T)的抑菌活性均不显著。综上所述，这些结果表明益生菌干酪乳杆菌Shirota菌株是一种有效的UTI治疗剂。
The antimicrobial activity of the intraurethrally administered probiotic Lactobacillus casei strain Shirota against Escherichia coli in a murine urinary tract infection (UTI) model was examined. UTI was induced by intraurethral administration of Escherichia coli strain HU-1 (a clinical isolate from a UTI patient, positive for type 1 and P fimbriae), at a dose of 1 x 10(6) to 2 x 10(6) CFU in 20 mul of saline, into a C3H/HeN mouse bladder which had been traumatized,vith 0.1 N HCl followed immediately by neutralization with 0.1 N NaOH 24 h before the challenge infection. Chronic infection with the pathogen at 106 CFU in the urinary tract (bladder and kidneys) was maintained for more than 3 weeks after the challenge, and the number of polymorphonuclear leukocytes and myeloperoxidase activity in the urine were markedly elevated during the infection period. A single administration oft. casei Shirota at a dose of 10(8) CFU 24 h before the challenge infection dramatically inhibited E. coli growth and inflammatory responses in the urinary tract. Multiple daily treatments with L. casei Shirota during the postinfection period also showed antimicrobial activity in this Un model, A heat-killed preparation of L. casei Shirota exerted significant antimicrobial effects not only with a single pretreatment (100 mug/mouse) but also with multiple daily treatments during the postinfection period. The other Lactobacillus strains tested, i.e., L. fermentum ATCC 14931(T), L. jensenii ATCC 25258(T), L. plantarum ATCC 14917(T), and L. reuteri JCM 1112(T), had no significant antimicrobial activity. Taken together, these results suggest that the probiotic L. casei strain Shirota is a potent therapeutic agent for UTI.