Quantitative analysis of major histocompatibility complex class II-positive cells in posterior segment of Royal College of Surgeons rat eyes
Quantitative analysis of major histocompatibility complex class II-positive cells in posterior segment of Royal College of Surgeons rat eyes
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DOI：
10.1016/s0021-5155(98)00035-5
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发表时间：
1998-09-01
影响因子：
2.4
通讯作者：
Tamai, M
中科院分区：
文献类型：
--
作者：
Akaishi, K;Ishiguro, S;Tamai, M
对RCS大鼠眼后段的潜在抗原呈递细胞进行了定量分析。在出生后10、20、28天进行光镜免疫组化。在RCS大鼠和它们的基因对应物的眼睛中有42、63和140。免疫组化研究使用单克隆抗体对抗主要组织相容性复合体（MHC） II类抗原（OX6）、骨髓源性巨噬细胞中的细胞质抗原（ED1）、驻存组织巨噬细胞的膜抗原（ED2）和小胶质细胞/巨噬细胞标记物（OX42）。用这些抗体对部分切片进行双标记染色。P10时，RCS大鼠视网膜未见MHCⅱ类阳性细胞。然而，它们在P20的外核层和外段碎片中被发现。从P20到P42细胞数先增加后减少，直至P140。然而，同源对照显示视网膜中没有MHC ii类阳性细胞。P42时外核层中存在OX6和ED1双标记的细胞，但未检测到OX6和OX42双标记的细胞。同时，未检测到ed2阳性细胞。我们的研究结果提示MHC ii类阳性细胞可能在视网膜营养不良中起一定作用。中华眼科杂志1998；[42]中国眼科杂志。
Potential antigen-presenting cells in the posterior segment of Royal College of Surgeons (RCS) rat eyes were analyzed quantitatively. Light microscopic immunohistochemistry was performed at postnatal days (P) 10, 20, 28. 42, 63, and 140 in the eyes of RCS rats and their congenic counterparts. Immunohistochemical studies were carried out using monoclonal antibodies against major histocompatibility complex (MHC) class II antigen (OX6), a cytoplasmic antigen in bone marrow-derived macrophages (ED1), a membrane antigen on resident tissue macrophages (ED2.), and a microglia/macrophage marker (OX42). Some sections were stained by a double-labeling method using these antibodies. No MHC class II-positive cells were seen in dystrophic RCS rat retinas at P10. They were found, however, in the outer nuclear layer and debris of outer segments at P20. From P20 to P42 the number of cells increased, then decreased until P140. Congenic controls, however, showed no MHC class II-positive cells in the retina. Cells double-labeled with OX6 and ED1 were present in the outer nuclear laver at P42, but no OX6 or OX42 double-labeled cells were detected. Also, no ED2-positive cells were detected. Our results suggest that MHC class II-positive cells may play some role in retinal dystrophy. Jpn J Ophthalmol 1998; 42:357-362 (C) 1998 Japanese Ophthalmological Society.