Detection and serotyping of Actinobacillus actinomycetemcomitans isolates on nitrocellulose paper blots with monoclonal antibodies.
Detection and serotyping of Actinobacillus actinomycetemcomitans isolates on nitrocellulose paper blots with monoclonal antibodies.
复制标题
使用单克隆抗体对硝酸纤维素纸印迹上的放线放线杆菌分离株进行检测和血清分型。
DOI：
10.1111/j.1600-051x.1986.tb00866.x
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发表时间：
1986
影响因子：
6.7
通讯作者：
Wilson,L
中科院分区：
文献类型：
--
作者：
McArthur,WP;Stroup,S;Wilson,L
用硝酸纤维素纸片上的细菌菌落印迹和特异性单克隆抗体结合抗原的免疫酶检测相结合的方法，直接从初始琼脂培养皿上检测放线菌伴放线杆菌。西娅放线菌共生菌抗原，代表特定的菌落，使用对种特异性抗原特异性的单克隆抗体进行免疫酶法鉴定。通过将印迹纸分成切片并使用血清型特异性单克隆抗体免疫酶法鉴定血清型抗原，鉴定菌落中细菌的血清型。该方法提供了简单、快速、灵敏和准确的鉴定和表征A。放线菌共生菌从初始分离琼脂平板分离。
A combination of blotting of bacterial colonies on nitrocellulose paper discs and immunoenzymatic detection of bound antigens with specific monoclonal antibodies was used to detect and serotypeActinobacillus actinomycetemcomitansdirectly off the initial agar culture dish. TheA. actinomycetemcomitansantigens, representative of specific colonies, were identified immunoenzymatically using monoclonal antibody specific for a species‐specific antigen. The serotype of the bacteria in colonies was identified by dividing the blotting paper into sections and immunoenzymatically identifying the serotype antigens with serotype‐specific monoclonal antibodies. This procedure provides for simple, rapid, sensitive and accurate identification and characterization ofA. actinomycetemcomitansisolates from the initial isolation agar plates.