CHD1 remodelers regulate nucleosome spacing in vitro and align nucleosomal arrays over gene coding regions in S. pombe
CHD1 remodelers regulate nucleosome spacing in vitro and align nucleosomal arrays over gene coding regions in S. pombe
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DOI：
10.1038/emboj.2012.289
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发表时间：
2012-11-28
期刊：
影响因子：
11.4
通讯作者：
Korber, Philipp
中科院分区：
文献类型：
--
作者：
Pointner, Julia;Persson, Jenna;Korber, Philipp
核小体定位控制着进入真核基因组。许多基因在其5 '端显示出刻板的组织：转录起始位点（TSS）上游的无核小体区，随后是编码区上方的规则核小体阵列。这种模式的决定因素尚不清楚，但核小体重塑可能是关键。在这里，我们研究的作用，重塑全球核小体定位在S。粟酒和相应的表达变化。我们发现了一个显着的进化转变，在重塑芽殖和裂变酵母之间的使用。色葡萄粟酒裂殖酵母RSC复合物似乎不参与核小体定位，尽管它在S.啤酒。而S.粟酒裂殖酵母缺乏ISWI型重塑蛋白，它有两个CHD 1型ATP酶，Hrp 1和Hrp 3。我们证明核小体间距活性Hrp 1和Hrp 3在体外，它们一起是必不可少的连接规则的基因阵列，大多数TSS在体内。在没有一个或两个重塑剂的情况下受损的阵列可能导致增加的隐蔽反义转录，但总体基因表达水平仅受到轻度影响。The EMBO Journal（2012）31，4388-4403. doi：10.1038/doj.2012.289; 2012年10月26日在线发布
Nucleosome positioning governs access to eukaryotic genomes. Many genes show a stereotypic organisation at their 5'end: a nucleosome free region just upstream of the transcription start site (TSS) followed by a regular nucleosomal array over the coding region. The determinants for this pattern are unclear, but nucleosome remodelers are likely critical. Here we study the role of remodelers in global nucleosome positioning in S. pombe and the corresponding changes in expression. We find a striking evolutionary shift in remodeler usage between budding and fission yeast. The S. pombe RSC complex does not seem to be involved in nucleosome positioning, despite its prominent role in S. cerevisiae. While S. pombe lacks ISWI-type remodelers, it has two CHD1-type ATPases, Hrp1 and Hrp3. We demonstrate nucleosome spacing activity for Hrp1 and Hrp3 in vitro, and that together they are essential for linking regular genic arrays to most TSSs in vivo. Impaired arrays in the absence of either or both remodelers may lead to increased cryptic antisense transcription, but overall gene expression levels are only mildly affected. The EMBO Journal (2012) 31, 4388-4403. doi:10.1038/emboj.2012.289; Published online 26 October 2012