CHANGES IN OXYTOCIN IMMUNOREACTIVITY AND MESSENGER-RNA EXPRESSION IN THE SHEEP BRAIN DURING PREGNANCY, PARTURITION AND LACTATION AND IN RESPONSE TO ESTROGEN AND PROGESTERONE
CHANGES IN OXYTOCIN IMMUNOREACTIVITY AND MESSENGER-RNA EXPRESSION IN THE SHEEP BRAIN DURING PREGNANCY, PARTURITION AND LACTATION AND IN RESPONSE TO ESTROGEN AND PROGESTERONE
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DOI：
10.1111/j.1365-2826.1993.tb00505.x
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发表时间：
1993-08-01
影响因子：
3.2
通讯作者：
KEVERNE, EB
中科院分区：
文献类型：
--
作者：
BROAD, KD;KENDRICK, KM;KEVERNE, EB
研究了妊娠、分娩和哺乳以及外源性雌二醇和孕酮处理对绵羊脑内催产素（OXY）免疫反应性和基因表达的影响。用免疫细胞化学方法观察室旁核（PVN）、视上核（SON）、终纹床核（BNST）、前连合核（ACN）和室周内侧视前区（PvMP）的细胞中OXY的表达。在副嗅核、嗅球球层和球周层、侧隔、未定带和黑质致密部也可见催产素免疫反应终末。与卵巢切除和晚期妊娠动物相比，在分娩时、哺乳期间和雌二醇外源性治疗后，所有这些催产素能元素的免疫反应强度均增加。OXY-免疫反应性也更强烈的晚期妊娠动物相比，卵巢切除的。定量原位杂交组织化学显示，在PVN，SON，BNST和PvMP的细胞都表现出显着增加的OXY mRNA的表达在分娩和哺乳期的动物相比，晚期妊娠或卵巢切除的动物。晚期妊娠动物的表达水平也显著高于卵巢切除动物。孕酮治疗显著增加PVN、SON、BNST和PvMP中的OXY mRNA，而雌二醇治疗仅在PVN、BNST和PvMP中有效。与这些类固醇的联合治疗没有显着增加OXY mRNA水平相比，他们的administrations.These结果表明，OXY-免疫反应性和mRNA的表达是在其最高的绵羊大脑时，母亲的行为是诱导。分娩时肽的合成/储存增加可能是由于孕晚期孕酮和雌二醇循环浓度的变化。
The effects of pregnancy, parturition and lactation and exogenous treatments with oestradiol and progesterone on oxytocin (OXY) immunoreactivity and gene expression in the sheep brain were investigated. Immunocytochemistry was used to demonstrate that increased OXY-immunoreactivity occurred in cells of the paraventricular (PVN) and supraoptic nuclei (SON), the bed nucleus of the stria terminalis (BNST), the anterior commissural nuclei (ACN) and the periventricular part of the medial preoptic area (PvMP). Oxytocin immunoreactive terminals were also seen in the accessory olfactory nucleus, the glomerular and peri-glomerular layers of the olfactory bulb, the lateral septum, the zona incerta and the pars compacta of the substantia nigra. Compared to ovariectomized and late pregnant animals, the intensity of immunoreactivity was increased in all of these oxytocinergic elements at parturition, during lactation and following exogenous treatment with oestradiol. The OXY-immunoreactivity was also more intense in late pregnant animals compared to ovariectomized ones. Quantitative in situ hybridization histochemistry showed that cells in the PVN, SON, BNST and PvMP all showed significantly increased expression of OXY mRNA in animals at parturition and during lactation compared to late pregnant or ovariectomized animals. Expression levels in late pregnant animals were also significantly higher than in ovariectomized ones. Progesterone treatment significantly increased OXY mRNA in the PVN, SON, BNST and PvMP whereas oestradiol treatment was only effective in the PVN, BNST and PvMP. Combined treatment with these steroids did not significantly increase OXY mRNA levels in comparison with their administration alone.These results show that OXY-immunoreactivity and mRNA expression are at their highest in the sheep brain when maternal behaviour is induced. The increased synthesis/storage of the peptide at parturition may be due to changes in circulating concentrations of both progesterone and oestradiol during late pregnancy.