Advances in the formation, use and understanding of multi-cellular spheroids.
Advances in the formation, use and understanding of multi-cellular spheroids.
复制标题
DOI：
10.1517/14712598.2012.707181
复制
发表时间：
2012-10
影响因子：
4.6
通讯作者：
Morgan JR
中科院分区：
文献类型：
--
作者：
Achilli TM;Meyer J;Morgan JR
开发用于研究细胞生物学和细胞生理学的体外模型对于生物技术、癌症研究、药物发现、毒性测试以及新兴的组织工程和再生医学领域都具有重要意义。哺乳动物细胞培养的传统二维（2D）方法具有若干限制，并且越来越多地认识到，由于增加的细胞间相互作用，并且通过模拟天然器官和组织的体内结构，在三维（3D）环境中生长的细胞更接近地代表正常细胞功能。在这篇综述中，我们讨论了形成三维多细胞球体的方法，这些方法的优点和局限性，以及用于表征球体功能的检测方法。球状体的使用导致了基础细胞科学的许多进步，包括理解癌细胞相互作用，创建药物发现和癌症转移的模型，并且它们正在被研究作为工程组织构建的基本单元。因此，本次审查将集中在使用球体模型对这些领域的贡献。多细胞球体具有丰富的生物学内容物，并且比2D细胞培养更好地模拟体内环境。形成和分析球体的新技术正在迅速增加其采用并扩大其应用。
Developing in vitro models for studying cell biology and cell physiology is of great importance to the fields of biotechnology, cancer research, drug discovery, toxicity testing, as well as the emerging fields of tissue engineering and regenerative medicine. Traditional two dimensional (2D) methods of mammalian cell culture have several limitations and it is increasingly recognized that cells grown in a three dimensional (3D) environment more closely represent normal cellular function due to the increased cell-to-cell interactions, and by mimicking the in vivo architecture of natural organs and tissues. In this review, we discuss the methods to form 3D multi-cellular spheroids, the advantages and limitations of these methods, and assays used to characterize the function of spheroids. The use of spheroids has led to many advances in basic cell sciences, including understanding cancer cell interactions, creating models for drug discovery and cancer metastasis, and they are being investigated as basic units for engineering tissue constructs. As so, this review will focus on contributions made to each of these fields using spheroid models. Multi-cellular spheroids are rich in biological content and mimic better the in vivo environment than 2D cell culture. New technologies to form and analyze spheroids are rapidly increasing their adoption and expanding their applications.
登录
查看更多内容
影响因子：
4.8
作者：
Bao, Brian A.;Lai, Charles P.;Morgan, Jeffrey R.
通讯作者：
Morgan, Jeffrey R.
影响因子：
--
作者：
Fukuda, J;Nakazawa, K
通讯作者：
Nakazawa, K
影响因子：
3
作者：
Frith, Jessica E.;Thomson, Brian;Genever, Paul G.
通讯作者：
Genever, Paul G.
影响因子：
--
作者：
Griffith, CK;Miller, C;George, SC
通讯作者：
George, SC
影响因子：
6.3
作者：
Chen, Nan;Glazier, James A.;Alber, Mark S.
通讯作者：
Alber, Mark S.