Quantitative flow cytometric evaluation of CD200, CD123, CD43 and CD52 as a tool for the differential diagnosis of mature B-cell neoplasms.
Quantitative flow cytometric evaluation of CD200, CD123, CD43 and CD52 as a tool for the differential diagnosis of mature B-cell neoplasms.
复制标题
CD200，CD123，CD43和CD52的定量流式细胞仪评估是成熟B细胞肿瘤的鉴别诊断的工具。
DOI：
10.1016/j.bjhh.2017.05.002
复制
发表时间：
2017-07
影响因子：
--
通讯作者：
Faulhaber GAM
中科院分区：
文献类型：
--
作者：
Arlindo EM;Marcondes NA;Fernandes FB;Faulhaber GAM
由于免疫特征和临床表现的重叠，成熟b细胞肿瘤很难区分。本研究探讨了在流式细胞仪面板上定量四种单克隆抗体的平均荧光强度是否有助于这些疾病的鉴别诊断和表征。分析了124例成熟b细胞肿瘤标本中CD52、CD200、CD123和CD43的表达情况。这些抗原的定量估计是通过平均荧光强度评估。其中慢性淋巴细胞白血病78例，非典型慢性淋巴细胞白血病3例，边缘带淋巴瘤6例，脾边缘带淋巴瘤11例，淋巴浆细胞淋巴瘤9例，套细胞淋巴瘤6例，毛细胞白血病2例，毛细胞白血病变异型2例，滤泡性淋巴瘤5例，伯基特淋巴瘤1例，弥漫性大b细胞淋巴瘤1例。CD200的平均荧光强度在非典型慢性淋巴细胞白血病、慢性淋巴细胞白血病和毛细胞白血病中较高。CD123在毛细胞白血病细胞中表现出较高的平均荧光强度。慢性淋巴细胞白血病、非典型慢性淋巴细胞白血病和套细胞淋巴瘤CD43的表达较高，滤泡性淋巴瘤的平均荧光强度值都很低。CD52在所有病例中均呈阳性表达。定量评价这些标志物可以作为一种有用的附加工具，以更好地识别某些类型的成熟b细胞肿瘤。
Distinction between mature B-cell neoplasms can be difficult due to overlapping of immunologic features and clinical manifestations. This study investigated whether quantifying mean fluorescence intensity of four monoclonal antibodies in a flow cytometry panel is useful for the differential diagnosis and characterization of these disorders. The expressions of CD52, CD200, CD123 and CD43 were analyzed in samples from 124 patients with mature B-cell neoplasms. The quantitative estimation of these antigens was assessed by mean fluorescence intensity. The cases included were 78 chronic lymphocytic leukemias, three atypical chronic lymphocytic leukemias, six marginal zone lymphomas, 11 splenic marginal zone lymphomas, nine lymphoplasmacytic lymphomas, six mantle cell lymphomas, two hairy cell leukemias, two hairy cell leukemias variant, five follicular lymphomas, one Burkitt lymphoma and one diffuse large B-cell lymphoma. The mean fluorescence intensity of CD200 was higher in atypical chronic lymphocytic leukemia, chronic lymphocytic leukemia and hairy cell leukemia cases. CD123 showed higher mean fluorescence intensities in hairy cell leukemia cells. Chronic lymphocytic leukemia, atypical chronic lymphocytic leukemia and mantle cell lymphoma had higher expression of CD43 and all follicular lymphoma cases had very low mean fluorescence intensity values. CD52 expression was consistently positive among all cases. Quantitative evaluation of these markers can be a useful additional tool to better identify some types of mature B-cell neoplasms.