Modular process for the flexible synthesis of magnetic beads—Process and product validation
Modular process for the flexible synthesis of magnetic beads—Process and product validation
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磁珠灵活合成的模块化工艺——工艺和产品验证
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
U. Peuker
中科院分区：
文献类型：
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作者：
B. Hickstein;U. Peuker
在这里，我们提出了一种模块化工艺，用于灵活地生产具有可变磁性和阴阳离子交换功能的磁珠。磁珠用于生物分离和下游加工，以从发酵液中去除有价值的物质(磁性捕鱼)。我们在这里介绍的磁珠由一种复合材料组成，其中包含纳米级的磁铁矿和嵌入到聚合物基质中的离子交换器颗粒。有了这种复合概念，含有不同基质聚合物(PVB、PVA、PMMA和PVAc)的磁珠具有阴离子和阳离子交换性能。磁铁矿的含量在0-40wt%之间变化，离子交换颗粒在0-60wt%之间变化，基质聚合物在20-60wt%之间变化。图中显示了决定应用性能的磁珠特性。热重分析、红外光谱和离子交换容量的测量证明了不同组成的磁珠具有不同的性质。在吸附实验中，β-半乳糖苷酶的最大吸附容量可达270 mg/g。©2009 Wiley期刊，Inc.《应用理工学院》，2009
Here we present a modular process for the flexible production of magnetic beads with variable magnetic properties and anion or cation exchanger functionalities. Magnetic beads are used in bio-separations and downstream processing to remove a valuable substance from e.g., a fermentation broth (Magnetic Fishing). The magnetic beads we are presenting here consist of a composite material, containing nanoscale magnetite and ion exchanger particles which are embedded into a polymer matrix. With this composite concept, anion and cation exchanger properties are available in magnetic beads with different matrix polymers (PVB, PVA, PMMA, and PVAc). The content of magnetite was varied in a range between 0 and 40 wt %, ion exchanger particles between 0 and 60 wt % and the matrix polymer between 20 and 60 wt %. The magnetic bead characteristics, which determine the application properties, are shown. Thermo gravimetrical analyses, FTIR spectra and measurements of the ion exchange capacity prove the different properties in respect to the magnetic beads composition. In an adsorption experiment, it was possible to achieve a maximum capacity of 270 mg/g for β-galactosidase with PVB-beads. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009