Plasma creatinine determination in mice and rats: An enzymatic method compares favorably with a high-performance liquid chromatography assay
Plasma creatinine determination in mice and rats: An enzymatic method compares favorably with a high-performance liquid chromatography assay
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DOI：
10.1038/sj.ki.5001988
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发表时间：
2007-01-01
影响因子：
19.6
通讯作者：
Pill, J.
中科院分区：
文献类型：
--
作者：
Keppler, A.;Gretz, N.;Pill, J.
使用比色法Jaffe法测量小鼠和大鼠血浆中的肌酐一直受到批评，因为先前的研究表明高估了很多。我们比较了苦味酸比色法、酶促法和高效液相色谱(HPLC法)三种方法在常规测定健康和疾病小鼠(n=61)和大鼠(n=56)血浆肌酸的适用性。对于色度学的Jaffe方法，确认了明显的高估。此外，该方法对大鼠血浆中已经处于非常低的、不可见的浓度范围内的血红蛋白进行了干扰。酶学测量表明，只有在溶血可见的情况下，小鼠的血红蛋白才会受到干扰。用高效液相色谱(HPLC法)和酶标法进行比较，两种方法在两种植物中的检测结果一致。因此，该酶法可满足在较宽的浓度范围内对健康和患病小鼠和大鼠的血浆肌酐进行常规筛选的要求。
The use of the colorimetric Jaffe method for the measurement of creatinine in mouse and rat plasma has been criticized as prior studies have shown a dramatic overestimation. We compared a colorimetric picric acid, an enzymatic, and a high-performance liquid chromatography (HPLC) method to assess their appropriateness for routine measurements of creatinine in plasma of healthy and diseased mice (n = 61) and rats (n = 56). For the colorimetric Jaffe method a pronounced overestimation is confirmed. Additionally the method showed interference with hemoglobin already in a very low, non-visible concentration range in rat plasma. The enzymatic measurement demonstrated a hemoglobin interference in mice, only when hemolysis was visible. The comparison between HPLC and the enzymatic measurement gave a good agreement between both methods in both species. Therefore the enzymatic method fulfills the requirements for a routine screening test for plasma creatinine in healthy as well as diseased mice and rats over a broad concentration range.