The indicative function of Twist2 and E-cadherin in HPV oncogene-induced epithelial-mesenchymal transition of cervical cancer cells
The indicative function of Twist2 and E-cadherin in HPV oncogene-induced epithelial-mesenchymal transition of cervical cancer cells
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Twist2和E-cadherin在HPV癌基因诱导的宫颈癌细胞上皮间质转化中的指示作用。
DOI：
10.3892/or.2014.3620
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发表时间：
2015-02-01
期刊：
影响因子：
4.2
通讯作者：
Wu, Sufang
中科院分区：
文献类型：
--
作者：
Liu, Yuan;Qian, Wenyan;Wu, Sufang
高危型人乳头瘤病毒(HR-HPV)感染是宫颈癌发生的重要因素之一。然而，感染HR-HPV的患者是否最终发展为恶性肿瘤，很大程度上取决于上皮-间充质转化(EMT)，它在肿瘤的发生和转移过程中发挥着非凡的作用。因此，我们用组织芯片和免疫组织化学方法检测了正常宫颈鳞状上皮、宫颈上皮内瘤变(CIN)和宫颈鳞癌(SCC)中EMT相关基因的蛋白水平。通过比较31例宫颈肿瘤及癌旁组织中EMT相关蛋白的表达情况，探讨HPV16癌基因与宫颈上皮内瘤变的关系，发现宫颈上皮内瘤变和宫颈癌组织中Twist2蛋白水平显著高于正常宫颈鳞状上皮组织(P<0.05)。
High-risk human papillomavirus (HR-HPV) infections are among the most important factors for cervical carcinogenesis. However, whether patients infected with HR-HPV eventually develop a malignant tumor, largely depends on epithelial-mesenchymal transition (EMT), which plays an extraordinary role in the process of carcinogenesis and metastasis. Therefore, we evaluated the protein levels of EMT-related genes in normal cervical squamous epithelium, cervical intraepithelial neoplasia (CIN), and cervical squamous cell carcinoma (SCC) by tissue microarray and immunohistochemical staining. By comparing the expression of EMT-related proteins in 31 cases of cervical tumors and tumor adjacent tissues and exploring the relationship between HPV16 oncogenes and EMT in vitro, we found that Twist2 protein levels were significantly higher in CIN and cervical cancer than in normal cervical squamous epithelial samples (p