Arabidopsis Rad51B is important for double-strand DNA breaks repair in somatic cells
Arabidopsis Rad51B is important for double-strand DNA breaks repair in somatic cells
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DOI：
10.1007/s11103-005-2187-1
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发表时间：
2005-04
影响因子：
5.1
通讯作者：
K. Osakabe;K. Abe;H. Yamanouchi;Toshio Takyuu;T. Yoshioka;Yuji Ito;Tomohiko Kato;S. Tabata;
中科院分区：
文献类型：
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作者：
K. Osakabe;K. Abe;H. Yamanouchi;Toshio Takyuu;T. Yoshioka;Yuji Ito;Tomohiko Kato;S. Tabata;
RAD51类似物属于Rad52上位性蛋白质组，参与同源重组，特别是RAD51的组装和稳定，RAD51是真核生物中RecA的同源物。我们先前克隆并鉴定了拟南芥中两个RAD51同源基因，分别命名为AtRAD51C和AtXRCC3，它们分别被认为是人类RAD51C和XRCC3的同源基因。本文描述了拟南芥中RAD51B同源基因AtRAD51B的鉴定。我们发现AtRAD51Bin在花芽和根中有较高的表达。AtRAD51B的表达被电离辐射和交联剂顺铂处理等遗传毒性应激诱导。酵母双杂交分析表明，AtRad51B与AtRad51C相互作用。我们还发现并鉴定了T-DNA插入突变系。突变株系无AtRAD51B的表达，有活力和可育性。突变株对γ-射线中度敏感，对顺铂高度敏感。我们的结果表明AtRAD51B基因产物参与了双链DNA断裂(DSB)的修复
Rad51 paralogs belong to the Rad52 epistasis group of proteins and are involved in homologous recombination (HR), especially the assembly and stabilization of Rad51, which is a homolog of RecA in eukaryotes. We previously cloned and characterized twoRAD51paralogous genes inArabidopsis, namedAtRAD51CandAtXRCC3, which are considered the counterparts of humanRAD51CandXRCC3, respectively. Here we describe the identification ofRAD51Bhomologue inArabidopsis,AtRAD51B. We found a higher expression ofAtRAD51Bin flower buds and roots. Expression ofAtRAD51Bwas induced by genotoxic stresses such as ionizing irradiation and treatment with a cross-linking reagent, cisplatin. Yeast two-hybrid analysis showed that AtRad51B interacted with AtRad51C. We also found and characterized T-DNA insertion mutant lines. The mutant lines were devoid ofAtRAD51Bexpression, viable and fertile. The mutants were moderately sensitive to γ-ray and hypersensitive to cisplatin. Our results suggest thatAtRAD51Bgene product is involved in the repair of double-strand DNA breaks (DSBs) via HR