The OprB porin plays a central role in carbohydrate uptake in Pseudomonas aeruginosa
The OprB porin plays a central role in carbohydrate uptake in Pseudomonas aeruginosa
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DOI：
10.1128/jb.177.11.3021-3026.1995
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发表时间：
1995-06
影响因子：
3.2
通讯作者：
John L. Wylie;E. Worobec
中科院分区：
文献类型：
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作者：
John L. Wylie;E. Worobec
利用内插子诱变，我们获得了缺乏或过表达底物选择性OprB孔蛋白的铜绿假单胞菌菌株。这些菌株最初对葡萄糖的摄取显著减少或增加，证实了OprB在促进葡萄糖通过铜绿假单胞菌外膜扩散方面的作用。然而，我们也证明了OprB的缺失或过表达对支持该细菌生长的其他三种糖(甘露醇、甘油和果糖)的吸收也有类似的影响。这种影响仅限于碳水化合物的运输；精氨酸摄取在突变株和野生型菌株中是相同的。这些结果表明，OprB不能被认为是严格意义上的葡萄糖选择性孔蛋白；相反，它是碳水化合物运输的中心成分，更准确地被描述为碳水化合物选择性孔蛋白。
Using interposon mutagenesis, we have generated strains of Pseudomonas aeruginosa which lack or overexpress the substrate-selective OprB porin of this species. A marked decrease or increase in the initial uptake of glucose by these strains verified the role of OprB in facilitating the diffusion of glucose across the outer membrane of P. aeruginosa. However, we also demonstrated that the loss or overexpression of OprB had a similar effect on the uptake of three other sugars able to support the growth of this bacterium (mannitol, glycerol, and fructose). This effect was restricted to carbohydrate transport; arginine uptake was identical in mutant and wild-type strains. These results indicated that OprB cannot be considered strictly a glucose-selective porin; rather, it acts as a central component of carbohydrate transport and is more accurately described as a carbohydrate-selective porin.