Resveratrol induces cervical cancer HeLa cell apoptosis through the activation and nuclear translocation promotion of FOXO3a
Resveratrol induces cervical cancer HeLa cell apoptosis through the activation and nuclear translocation promotion of FOXO3a
复制标题
白藜芦醇通过激活FOXO3a并促进核转位诱导宫颈癌HeLa细胞凋亡
DOI：
10.1691/ph.2020.0386
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发表时间：
2020-06-01
期刊：
影响因子：
1.6
通讯作者：
Sun, Yunxiao
中科院分区：
文献类型：
--
作者：
Liu, Zhixin;Li, Youjie;Sun, Yunxiao
白藜芦醇（反式-3，4 'V，5-三羟基芪）除了具有抗癌潜力外，还具有抗氧化、抗炎和心脏保护功能。在这项研究中，我们探讨了白藜芦醇，作为一种抗癌剂，如何有效地影响宫颈癌HeLa细胞。MTT比色法和流式细胞仪检测结果显示，白藜芦醇能显著抑制HeLa细胞增殖，诱导其凋亡。免疫荧光染色结果提示白藜芦醇可促进FOXO 3a核转位。我们重点研究了白藜芦醇促进HeLa细胞凋亡的机制。以下实验表明，可能的初始机制涉及Forkhead box O（FOXO）3a表达的上调，这进一步增加了Bcl-2相互作用的细胞死亡介导因子（BIM）的表达，该基因在凋亡中转录。白藜芦醇还可降低细胞外信号调节激酶（ERK）的活性，激活FOXO 3a，导致HeLa细胞凋亡。总之，这两种机制刺激激活FOXO 3a的积累，促进其核转位，并最终导致HeLa细胞凋亡。因此，白藜芦醇可能具有治疗宫颈癌的潜力。
Resveratrol (trans-3,4'V,5-trihydroxystilbene) presents antioxidant, anti-inflammatory, and cardioprotective functions in addition to its anticancer potential. In this study, we explored how resveratrol, as an anticancer agent, effectively influences cervical cancer HeLa cells. Our data showed that resveratrol could significantly inhibit HeLa cell proliferation and induce their apoptosis, as measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay and flow cytometry. The immunofluorescence staining results in the present study suggested that resveratrol could facilitate FOXO3a nuclear translocation. We then focused on the mechanism of resveratrol in promoting HeLa cell apoptosis. The following experiments suggested that the possible initial mechanism involves the upregulation Forkhead box O (FOXO) 3a expression, which further increases the expression of Bcl-2 interacting mediator of cell death (BIM), the gene transcribed in apoptosis. Resveratrol could also inactivate the basal extracellular signal-regulated kinase (ERK) activity, causing FOXO3a activation and resulting in HeLa cell apoptosis. In summary, both mechanisms stimulated the accumulation of activated FOXO3a, promoted its nuclear translocation, and ultimately caused HeLa cell apoptosis. Thus, resveratrol may have a potential in the treatment of cervical cancer.