Differences in leaf proteome response to cold acclimation between Lolium perenne plants with distinct levels of frost tolerance
Differences in leaf proteome response to cold acclimation between Lolium perenne plants with distinct levels of frost tolerance
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DOI：
10.1016/j.jplph.2011.01.029
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发表时间：
2011-07-15
影响因子：
4.3
通讯作者：
Zwierzykowski, Zbigniew
中科院分区：
文献类型：
--
作者：
Bocian, Aleksandra;Kosmala, Arkadiusz;Zwierzykowski, Zbigniew
多年生黑麦草（Lolium perenne）因其优良的营养价值和快速的生长速度而成为优质饲料和草坪草。然而，该物种在恶劣的冬季气候下的表现能力有限。尽管耐寒性是一个复杂的特性，但人们普遍认为耐冻性（FT）是其主要组成部分。在温带地区生长的物种可以通过暴露在非致命的低温下获得 FT，这种现象称为冷驯化 (CA)。 FT的分子基础研究已经在模式植物上进行，但它们不能很好地适应极端的冬季气候。因此，冬季作物和具有重要农艺意义的多年生草本植物细胞对低温的反应机制尚未被揭示。在这里，选择了两种具有对比 FT 水平的多年生植物，高耐霜（HFT）和低霜耐（LFT）植物进行比较蛋白质组研究。该工作重点是使用高通量二维电泳分析 CA 2、8、26 小时以及 3、5、7、14 和 21 天之前和之后的叶片蛋白质积累，并通过应用质谱 (MS) 鉴定所选植物之间差异积累的蛋白质。对 580 个蛋白质图谱的分析显示，总共 42 个 (7.2%) 点在 HFT 和 LFT 基因型之间的 CA 的一个时间点至少显示出 1.5 倍的蛋白质丰度差异。结果表明，所分析植物之间蛋白质积累谱的显着差异最常出现在 CA 的第 5 天（18 个蛋白质）和第 7 天（19 个蛋白质）。通过使用 MS 成功鉴定了来自 35 个点 (83.3%) 的蛋白质，并且叶绿体蛋白质被证明是在 CA 期间选择为差异积累的主要组。讨论了所鉴定蛋白质的功能及其对多年生黑麦草中 FT 水平的可能影响。 (C) 2011 爱思唯尔有限公司。版权所有。
Perennial ryegrass (Lolium perenne) is a high quality forage and turf grass mainly due to its excellent nutritive values and rapid establishment rate. However, this species has limited ability to perform in harsh winter climates. Though winter hardiness is a complex trait, it is commonly agreed that frost tolerance (FT) is its main component. Species growing in temperate regions can acquire FT through exposure to low, non-lethal temperatures, a phenomenon known as cold acclimation (CA). The research on molecular basis of FT has been performed on the model plants, but they are not well adapted to extreme winter climates. Thus, the mechanisms of cell response to low temperature in winter crops and agronomically important perennial grasses have yet to be revealed. Here, two L. perenne plants with contrasting levels of FT, high frost tolerant (HFT) and low frost tolerant (LFT) plants, were selected for comparative proteomic research. The work focused on analyses of leaf protein accumulation before and after 2, 8, 26 h, and 3, 5, 7, 14 and 21 days of CA, using a high-throughput two-dimensional electrophoresis, and on the identification of proteins which were accumulated differentially between the selected plants by the application of mass spectrometry (MS). Analyses of 580 protein profiles revealed a total of 42 (7.2%) spots that showed at a minimum of 1.5-fold differences in protein abundance, at a minimum of at one time point of CA between HFT and LFT genotypes. It was shown that significant differences in profiles of protein accumulation between the analyzed plants appeared most often on the 5th (18 proteins) and the 7th (19 proteins) day of CA. The proteins derived from 35 (83.3%) spots were successfully identified by the use of MS and chloroplast proteins were shown to be the major group selected as differentially accumulated during CA. The functions of the identified proteins and their probable influence on the level of FT in L perenne are discussed. (C) 2011 Elsevier GmbH. All rights reserved.