Keratinocyte derived T-cell growth factor (KTGF) is identical to granulocyte macrophage colony stimulating factor (GM-CSF).
Keratinocyte derived T-cell growth factor (KTGF) is identical to granulocyte macrophage colony stimulating factor (GM-CSF).
复制标题
角质形成细胞衍生的 T 细胞生长因子 (KTGF) 与粒细胞巨噬细胞集落刺激因子 (GM-CSF) 相同。
DOI：
10.1111/1523-1747.ep12464470
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Horowitz,M
中科院分区：
文献类型：
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作者：
Kupper,TS;Lee,F;Coleman,D;Chodakewitz,J;Flood,P;Horowitz,M
角质形成细胞衍生的T细胞生长因子最初被描述为培养的新生角质形成细胞和角质形成细胞系的产物，其诱导HT-2细胞增殖，HT-2细胞是通过掺入3 H-胸苷对IL-2和IL-4应答的鼠T细胞系。随后，KTGF已被纯化至高比活性，并通过多种生化、免疫学和免疫化学标准发现其不同于IL-2和IL-4。由于发现某些HT-2细胞系也响应于GM-CSF而增殖，因此本研究询问KTGF是否与GM-CSF相关。在这项研究中，我们证明了重组鼠GM-CSF的抗体完全中和KTGF诱导HT-2增殖的能力，而不干扰IL-2或IL-4诱导的HT-2增殖。Pam 212细胞中检测到与鼠GM-CSF cDNA同源的poly-A+ RNA，Pam 212条件培养液中检测到与GM-CSF血清学同源的蛋白质。我们得出结论，KTGF是相同的GM-CSF。GM-CSF的T细胞活化特性需要进一步探索。
Keratinocyte derived T-cell growth factor was initially described as a product of cultured neonatal keratinocytes and keratinocyte cell lines that induced the proliferation of HT-2 cells, a murine T-ceIl line that responds to IL-2 and IL-4 by incorporating 3H-Thymidine. Subsequently, KTGF has been purified to high specific activity and found to be distinct from IL-2 and IL-4 by a variety of biochemical, immunologic, and immunochemical criteria. Because it was found that certain HT-2 cell lines also proliferated in response to GM-CSF, the present study asked whether KTGF was related to GM-CSF. In this study, we demonstrate that antibodies to recombinant murine GM-CSF completely neutralize the capacity of KTGF to induce HT-2 proliferation without interfering with IL-2 or IL-4 induced HT-2 proliferation. Furthermore, poly-A+ RNA homologous to murine GM-CSF cDNA as judged by S1 nuclease analysis was detected in Pam 212 cells, and protein serologically homologous to GM-CSF was found in Pam 212 conditioned medium. We conclude that KTGF is identical to GM-CSF. The T-cell activating properties of GM-CSF require further exploration.