Lysophosphatidylethanolamina acyltransfcrase/membrane-bound O-acyltransferase 1 regulates morphology and function of P19C6 cell-derived neurons.
Lysophosphatidylethanolamina acyltransfcrase/membrane-bound O-acyltransferase 1 regulates morphology and function of P19C6 cell-derived neurons.
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溶血磷脂酰乙醇胺酰基转移酶/膜结合 O-酰基转移酶 1 调节 P19C6 细胞源性神经元的形态和功能。
DOI：
10.1096/fj.201500097r
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Tabe S， Hikiji H， Ariyoshi W， Hashidate-Yoshida T， Shindou H，Okinaga T， Shimizu T， Tominaga K，Nishihara T.
中科院分区：
文献类型：
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作者：
Tada H.;R. Suzuki;E. Nemoto;H. Shimauchi;K. Matushita;H. Takada.;Tabe S， Hikiji H， Ariyoshi W， Hashidate-Yoshida T， Shindou H，Okinaga T， Shimizu T， Tominaga K，Nishihara T.
甘油磷脂是生物膜的组成部分，由肯尼迪途径形成，随后通过Lands循环成熟。溶血磷脂酰基转移酶(LPLATs)是这两个途径中的关键酶，影响生物膜的脂肪酸组成。神经元分化的特点是突起生长，这需要生物膜的生物合成。然而，LPLATs在神经元分化中的作用仍不清楚。在这项研究中，我们使用全反式维甲酸(ATRA)处理的P19C6细胞来研究LPLATs是否参与神经元分化。在这些细胞中，溶血磷脂酰基乙醇胺酰基转移酶(LPEAT)-1/膜结合O-酰基转移酶(MBOAT)-1的mRNA水平高于未分化细胞。以16：0-CoA和18：1-CoA为酰基供体，LPEAT酶活提高。当用小干扰RNA(SiRNA)敲除LPEAT1/MBOAT1后，ATRA处理的P19C6细胞突起生长减少，神经元标志物表达减少。在这些细胞中，LPEAT1/MBOAT1 siRNA的电压依赖性钙通道活动也受到抑制。-Tabe，S.，Hikiji，H.，Ariyoshi，W.，Hashidate-Yoshida，T.，Shindou，H.，Okinaga，T.，Shimizu，T.，Tominaga，K.，Nishihara，T.FASE B J.30,2591-2601(2016)。Www.fasebj.org
Glycerophospholipids, which are components of biomembranes, are formedde novoby the Kennedy pathway and subsequently mature through the Lands cycle. Lysophospholipid acyltransferases (LPLATs) are key enzymes in both pathways and influence the fatty acid composition of biomembranes. Neuronal differentiation is characterized by neurite outgrowth, which requires biomembrane biosynthesis. However, the role of LPLATs in neuronal differentiation remains unknown. In this study, we examined whether LPLATs are involved in neuronal differentiation using all‐trans‐retinoic acid (ATRA)‐treated P19C6 cells. In these cells, mRNA levels of lysophosphatidylethanolamine acyltransferase (LPEAT)‐1/membrane‐boundO‐acyltransferase (MBOAT)‐1 were higher than those in undifferentiated cells. LPEAT enzymatic activity increased with 16:0‐ and 18:1‐CoA as acyl donors. When LPEAT1/MBOAT1 was knocked down with small interfering RNA (siRNA), outgrowth of neurites and expression of neuronal markers decreased in ATRA‐treated P19C6 cells. Voltage‐dependent calcium channel activity was also suppressed in these cells transfected with LPEAT1/MBOAT1 siRNA. These results suggest that LPEAT1/MBOAT1 plays an important role in neurite outgrowth and function.—Tabe, S., Hikiji, H., Ariyoshi, W., Hashidate‐Yoshida, T., Shindou, H., Okinaga, T., Shimizu, T., Tominaga, K., Nishihara, T. Lysophosphatidylethanolamine acyltransferase 1/membrane‐boundO‐acyltransferase 1 regulates morphology and function of P19C6 cell‐derived neurons. FASEB J. 30, 2591‐2601 (2016). www.fasebj.org