Plant regeneration via somatic embryogenesis of Elymus sibiricus cv. ‘chuancao No. 2’
Plant regeneration via somatic embryogenesis of Elymus sibiricus cv. ‘chuancao No. 2’
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DOI：
10.1007/s11240-005-9035-5
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发表时间：
2006-03
期刊：
影响因子：
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通讯作者：
Da-xu Li;Jie Zhang;Jian Zhao;Yi Zhang;Fei Chen;Jing-qiu Zhu;Shujun Liu;Zhi-rong Yang
中科院分区：
文献类型：
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作者：
Da-xu Li;Jie Zhang;Jian Zhao;Yi Zhang;Fei Chen;Jing-qiu Zhu;Shujun Liu;Zhi-rong Yang
本文报道了西伯利亚狗牙根的成熟种子、中胚轴和幼叶尖。CV.‘川草2号’在附加5.0mg/L 2，4-二氯苯氧乙酸（2，4-d）和0.05mg/L激动素的MS培养基上，于26 ℃黑暗条件下培养，产生愈伤组织。愈伤组织再生率与外植体来源和植物生长调节剂有关。继代培养8周后，白黄色致密的结节状愈伤组织形成再生植株。不同类型外植体的愈伤组织在含有半浓度MS盐+0.1mg/L激动素+1.5mg/L2，4-D +20g/L蔗糖的再生培养基上，分化率（54%）高于中胚轴（2,4%）和幼叶尖（6%）。绿色植物在根再生培养基中6周内生根，并且当盆栽在砂和泥炭混合物培养基中时，在温室中获得超过97%的这些土壤建立的植物。
The mature seeds, mesocotyls, and young leaf tips ofElymus sibiricusL. cv. ‘chuancao No. 2’ were cultured on Murashige and Skoog (MS) medium supplemented with 5.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-d) and 0.05 mg/L kinetin in the dark at 26°C, the calluses were produced. The rate of callus regeneration depended on the explants source and plant growth regulators. Plants regenerated from whitish-yellow-coloured compact nodular callus formed after subculturing for 8 weeks. Higher frequency (54%) of shoot differentiation was obtained from the embryo tissues of mature seed than from either mesocotyls (24%) or young leaf tip tissues (6%) when these calluses from different types of explants were cultured on plant regeneration medium containing half strength MS salts supplemented with 0.1 mg/L kinetin, 1.5 mg/L 2,4-Dand 20 g/L sucrose. The green plants were rooted within 6 weeks in the root regeneration medium, and over 97% of these soil-established plants were obtained in the greenhouse when potted in a sand and peat mixture medium.