POLYPRENOL REDUCTASE2 Deficiency Is Lethal in Arabidopsis Due to Male Sterility
POLYPRENOL REDUCTASE2 Deficiency Is Lethal in Arabidopsis Due to Male Sterility
复制标题
DOI：
10.1105/tpc.15.00463
复制
发表时间：
2015-12-01
期刊：
影响因子：
11.6
通讯作者：
Swiezewska, Ewa
中科院分区：
文献类型：
--
作者：
Jozwiak, Adam;Gutkowska, Malgorzata;Swiezewska, Ewa
Dolichol 是蛋白质糖基化所需的辅助因子，蛋白质糖基化是调节所有真核细胞中蛋白质稳定性和生物活性的最常见翻译后修饰。我们已经鉴定并表征了两个基因 PPRD1 和 -2，它们与人 SRD5A3（类固醇 5 α 还原酶 3 型）直系同源，并编码负责在拟南芥中将聚戊烯醇转化为多羟基化合物的聚戊烯醇还原酶。 PPRD1 和-2 在植物代谢中发挥着专门的作用。 PPRD2 对于植物活力至关重要；它的缺乏导致雄配子体和孢子体发育异常。蛋白质糖基化受损似乎是这些缺陷的主要因素，尽管其他细胞多醇依赖性过程的干扰也可能有所贡献。 PPRD2 缺陷细胞中多羟基化合物的短缺可以通过 PPRD1 过表达或补充多羟基化合物来部分缓解。后者被认为是一种补偿蛋白质糖基化缺陷的方法。在人类饮食中补充富含多甘醇的植物组织可以为糖基化疾病提供新的治疗干预措施。 PPRD1和-2的鉴定阐明了介导植物细胞中多醇循环关键步骤的因素，这使得操纵植物组织中多醇含量成为可能。
Dolichol is a required cofactor for protein glycosylation, the most common posttranslational modification modulating the stability and biological activity of proteins in all eukaryotic cells. We have identified and characterized two genes, PPRD1 and -2, which are orthologous to human SRD5A3 (steroid 5 alpha reductase type 3) and encode polyprenol reductases responsible for conversion of polyprenol to dolichol in Arabidopsis thaliana. PPRD1 and -2 play dedicated roles in plant metabolism. PPRD2 is essential for plant viability; its deficiency results in aberrant development of the male gametophyte and sporophyte. Impaired protein glycosylation seems to be the major factor underlying these defects although disturbances in other cellular dolichol-dependent processes could also contribute. Shortage of dolichol in PPRD2-deficient cells is partially rescued by PPRD1 overexpression or by supplementation with dolichol. The latter has been discussed as a method to compensate for deficiency in protein glycosylation. Supplementation of the human diet with dolichol-enriched plant tissues could allow new therapeutic interventions in glycosylation disorders. This identification of PPRD1 and -2 elucidates the factors mediating the key step of the dolichol cycle in plant cells which makes manipulation of dolichol content in plant tissues feasible.