Phenotypic and molecular characterization of antimicrobial resistance in Proteus mirabilis isolates from dogs
Phenotypic and molecular characterization of antimicrobial resistance in Proteus mirabilis isolates from dogs
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DOI：
10.1099/jmm.0.081539-0
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发表时间：
2014-11-01
影响因子：
3
通讯作者：
Kataoka, Yasushi
中科院分区：
文献类型：
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作者：
Harada, Kazuki;Niina, Ayaka;Kataoka, Yasushi
使用从日本犬中分离的103株奇异变形杆菌菌株对14种抗菌药物进行大规模监测。对耐药菌株进行了分析，以确定其耐药机制。对氯霉素、链霉素、恩诺沙星、甲氧苄啶/磺胺甲恶唑、卡那霉素、氨苄西林、环丙沙星、头孢噻吩、庆大霉素、头孢西丁和头孢噻肟的耐药率分别为20.4%、15.5%、12.6%、10.7%、9.7%、8.7%、5.8%、2.9%、2.9%、1.9%和1.9%。对头孢他啶、氨曲南和亚胺培南均未发现耐药，1类和2类整合酶检出率分别为2.9%和11.7%。1类整合子含有aadB或aadB-catB-like-bla（OXA 10）-aadA 1，而2类整合子含有sat aadA 1、dhfr 1-sat-aadA 1或不含预期的耐药基因。在5种不同的质粒介导的喹诺酮类耐药（PMQR）基因中，仅qnrD基因在1.9%的分离株中检出。对13株恩诺沙星中间型和耐药株的gyrA和parC基因的喹诺酮耐药决定区（CIRDRs）进行了测序。7株为双突变，3株为单突变。9株氨苄西林耐药分离株中有3株携带AmpC型β-内酰胺酶（即bla（CMY-2）、bla（CMY-4）和bla（DHA-1））。这些结果表明，犬奇异变形杆菌值得继续监测作为一个重要的水库抗菌素耐药决定因素。这是第一份报告，据我们所知，描述整合子，PMQRs和QRDR突变的奇异变形杆菌分离伴侣动物。
Large-scale monitoring of resistance to 14 antimicrobial agents was performed using 103 Proteus mirabilis strains isolated from dogs in Japan. Resistant strains were analysed to identify their resistance mechanisms. Rates of resistance to chloramphenicol, streptomycin, enrofloxacin, trimethoprim/sulfamethoxazole, kanamycin, ampicillin, ciprofloxacin, cephalothin, gentamicin, cefoxitin and cefotaxime were 20.4, 15.5, 12.6, 10.7, 9.7, 8.7, 5.8, 2.9, 2.9, 1.9 and 1.9%, respectively. No resistance to ceftazidime, aztreonam or imipenem was found. Class 1 and 2 integrases were detected in 2.9 and 11.7% of isolates, respectively. Class 1 integrons contained aadB or aadB-catB-like-bla(OXA10)-aadA1, whereas those of class 2 contained sat aadA 1, dhfr1-sat-aadA1 or none of the anticipated resistance genes. Of five distinct plasmid-mediated quinolone-resistance (PMQR) genes, only qnrD gene was detected in 1.9% of isolates. Quinolone-resistance determining regions (CIRDRs) of gyrA and parC from 13 enrofloxacin-intermediate and -resistant isolates were sequenced. Seven strains had double mutations and three had single mutations. Three of nine ampicillin-resistant isolates harboured AmpC-type beta-lactamases (i.e. bla(CMY-2), bla(CMY-4) and bla(DHA-1)). These results suggest that canine Proteus mirabilis deserves continued surveillance as an important reservoir of antimicrobial resistance determinants. This is the first report, to our knowledge, describing integrons, PMQRs and QRDR mutations in Proteus mirabilis isolates from companion animals.