Interactions between perchlorate and nitrate reductions in the biofilm of a hydrogen-based membrane biofilm reactor.
Interactions between perchlorate and nitrate reductions in the biofilm of a hydrogen-based membrane biofilm reactor.
复制标题
DOI：
10.1021/es202569b
复制
发表时间：
2011-11
影响因子：
11.4
通讯作者：
He-ping Zhao;Steven W. van Ginkel;Youneng Tang;Dae-Wook Kang;B. Rittmann;R. Krajmalnik-Brown
中科院分区：
文献类型：
--
作者：
He-ping Zhao;Steven W. van Ginkel;Youneng Tang;Dae-Wook Kang;B. Rittmann;R. Krajmalnik-Brown
研究了在氢气膜生物膜反应器（MBfR）中硝酸盐（NO（3）（-））和高氯酸盐（ClO（4）（-））还原的微生物功能和结构相互作用。当H（2）不受限制时，ClO（4）（-）和NO（3）（-）完全还原，并且MBfR的生物膜主要由来自ε-和β-变形菌纲的细菌组成，其中自养属Sulfuricurvum，Hydrogenophaga和Dechloromonas占主导地位。基于功能基因和焦磷酸测序分析，Dechloromonas在ClO（4）（-）还原中起主要作用，而Sulfuricurvum和Hydrogenophaga则在NO（3）（-）还原中起主要作用。当H（2）输送不足以完全还原两种电子受体时，NO（3）（-）还原竞争ClO（4）（-）还原来自H（2）的电子，并且混合营养菌在MBfR生物膜中变得重要。β-Proteobacteria成为优势类群，Azonexus取代Sulfuricurvum成为主要属。这些变化表明，兼性，NO（3）（-）-还原细菌具有优势，严格的自养时，H（2）是有限的，因为有机微生物产品成为重要的电子供体时，H（2）是严重限制。
We studied the microbial functional and structural interactions between nitrate (NO(3)(-)) and perchlorate (ClO(4)(-)) reductions in the hydrogen (H(2))-based membrane biofilm reactor (MBfR). When H(2) was not limiting, ClO(4)(-) and NO(3)(-) reductions were complete, and the MBfR's biofilm was composed mainly of bacteria from the ε- and β-proteobacteria classes, with autotrophic genera Sulfuricurvum, Hydrogenophaga, and Dechloromonas dominating the biofilm. Based on functional-gene and pyrosequencing assays, Dechloromonas played the most important role in ClO(4)(-) reduction, while Sulfuricurvum and Hydrogenophaga were responsible for NO(3)(-) reduction. When H(2) delivery was insufficient to completely reduce both electron acceptors, NO(3)(-) reduction out-competed ClO(4)(-) reduction for electrons from H(2), and mixotrophs become important in the MBfR biofilm. β-Proteobacteria became the dominant class, and Azonexus replaced Sulfuricurvum as a main genus. The changes suggest that facultative, NO(3)(-)-reducing bacteria had advantages over strict autotrophs when H(2) was limiting, because organic microbial products became important electron donors when H(2) was severely limiting.