Characterization of mononuclear blood cell-derived differentiation inducing factors for the human promyelocytic leukemia cell line HL-60.
Characterization of mononuclear blood cell-derived differentiation inducing factors for the human promyelocytic leukemia cell line HL-60.
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人早幼粒细胞白血病细胞系 HL-60 的单核血细胞衍生分化诱导因子的表征。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
N. Mauritzon
中科院分区：
文献类型：
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作者：
I. Olsson;T. Olofsson;N. Mauritzon
对人早幼粒细胞白血病HL-60细胞系进行了生理性分化诱导因子（DIF）的研究。单核血细胞在受到各种有丝分裂原的刺激时产生DIF，诱导HL-60分化为具有吞噬能力的硝基蓝四氮唑还原细胞，其形态特征为粒细胞或骨髓单核细胞。在含血清和无血清培养基中诱导分化的效果相同。蛋白质合成是精化DIF的必要条件，DIF似乎具有蛋白质性质，因为它被蛋白酶破坏。凝胶层析表明，根据使用的有丝分裂原的类型，可以产生一种或两种表观分子量为40,000和25,000的DIF。至少40,000分子量的DIF与同时产生的集落刺激活性（CSA）不同。我们的研究结果表明淋巴细胞和/或单核细胞在非CSA介导的骨髓单核细胞造血中起调节作用。然而，生理上的重要性仍有待证明。
A search was made for physiologically produced differentiation inducing factor(s) (DIF) for the human promyelocytic leukemia cell line HL-60. Mononuclear blood cells, when stimulated with various mitogens, produced DIF, which induced differentiation of HL-60 into phagocytizing nitro blue tetrazolium reducing cells with the morphologic characteristic of granulopoietic or myelomonocytic cells. Induction of differentiation occurred equally well in serum-containing and serum-free media. Protein synthesis was necessary for elaboration of DIF, which seems to be of a protein nature inasmuch as it is destroyed by proteases. Gel chromatography showed that one or two species of DIF with apparent molecular weights of 40,000 and 25,000 were produced, depending on the type of mitogen used. At least the 40,000-molecular weight DIF was distinct from the colony stimulating activity (CSA), which was produced simultaneously. Our results suggested a role of lymphocytes and/or monocytes for modulation of myelomonocytic hematopoiesis not mediated by CSA. The physiologic importance remains, however, to be demonstrated.