Antibacterial, Anti-Biofouling, and Antioxidant Prospects of Metal-Based Nanomaterials
Antibacterial, Anti-Biofouling, and Antioxidant Prospects of Metal-Based Nanomaterials
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DOI：
10.1002/clen.201500366
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发表时间：
2016-07-01
影响因子：
1.7
通讯作者：
Stalikas, Constantine D.
中科院分区：
文献类型：
--
作者：
Chatzimitakos, Theodoros;Kallimanis, Aristeidis;Stalikas, Constantine D.
三种金属纳米材料，使用湿化学方法容易地合成铁、铜和铁-铜，并适当地表征。针对两种细菌菌株，金黄色葡萄球菌和大肠杆菌测试材料，以评估其抗菌活性以及其生物膜形成抑制特性。在低mg/mL浓度下，细菌生长的总抑制意味着纳米材料对细菌具有令人满意的抗菌活性。两种菌株中，E. coli比S.金黄色。然而，由于S.金黄色葡萄球菌生物膜形成更容易被抑制。对两种不同细菌的作用方式存在差异，并提出了不同的作用机制来证明它们的合理性。此外，阳性1，1-二苯基-2-苦肼基（DPPH）自由基测试表明，纳米材料是自由基清除剂，而TBARS（硫代巴比妥酸反应物质）测试证明了其脂质氧化抑制特性，尽管浓度很高。铜纳米颗粒被证明是最有效的三种材料测试，对DPPH自由基和脂质过氧化反应，其次是铁-铜，最后由铁纳米颗粒。铁基材料的固有磁性可以为水净化系统中的抗菌或抗生物污垢处理以及抗氧化活性目的提供长期益处。
Three metallic nanomaterials, i.e., iron, copper, and bimetallic iron-copper were easily synthesized using a wet-chemical method and were properly characterized. The materials were tested against two bacterial strains, Staphylococcus aureus and Escherichia coli in order to evaluate their antibacterial activity, as well as their biofilm formation inhibition properties. The total inhibition of bacterial growth, at low mg/mL concentrations, implies the satisfactory antibacterial activity of the nanomaterials against bacteria. Between the two bacterial strains used, E. coli is more susceptible to the effect of the nanomaterials than S. aureus. However, the results for the respective biofilms are significantly different since S. aureus biofilm formation is more easily inhibited. There are differences in the way of action against the two different bacteria and diverse mechanisms of action are proposed to justify them. Furthermore, positive 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical tests demonstrated that the nanomaterials are free-radical scavengers while TBARS (thiobarbituric acid reactive substances) tests evidenced their lipid oxidation inhibition properties, although at high concentration levels. Copper nanoparticles proved to be the most effective of the three materials tested, against DPPH radicals and lipid peroxidation followed by the Fe-Cu and lastly by the Fe nanoparticles. The intrinsic magnetic properties of iron-based materials can provide long-term benefits for antibacterial or anti-biofouling treatment in water purification systems as well as for antioxidant activity purposes.