PROTECTION AGAINST FELINE INFECTIOUS PERITONITIS BY INTRANASAL INOCULATION OF A TEMPERATURE-SENSITIVE FIPV VACCINE
PROTECTION AGAINST FELINE INFECTIOUS PERITONITIS BY INTRANASAL INOCULATION OF A TEMPERATURE-SENSITIVE FIPV VACCINE
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DOI：
10.1016/0264-410x(90)90004-6
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发表时间：
1990-12-01
期刊：
影响因子：
5.5
通讯作者：
BECKENHAUER, WH
中科院分区：
文献类型：
--
作者：
GERBER, JD;INGERSOLL, JD;BECKENHAUER, WH
鼻内接种（i.n.）的猫用温度敏感的猫传染性腹膜炎病毒（ts-FIPV）疫苗接种的小鼠，可保护其免受FIP诱导的攻击。20只接种猫中有17只（85%）在强毒FIPV攻毒中存活，该攻毒导致12只未接种猫中有12只（100%）发生FIP，其中10只（83%）死亡。鼻内接种刺激血清IgG和血清和唾液伊加抗体应答（通过ELISA测量）、FIPV中和抗体（VN）和细胞介导的免疫（CMI）应答，如通过淋巴细胞增殖测量的。对疫苗接种的血清抗体应答与保护无关。事实上，攻毒后，对照猫的IgG、伊加和VN滴度远高于接种猫，表明存在免疫介导的发病机制。与此相反，刺激粘膜伊加的反应，疫苗接种有关的保护。在免疫猫、免疫猫和攻毒猫中观察到外周血淋巴细胞对强毒FIPV的体外增殖反应，但未在未免疫攻毒猫中观察到。
Cats vaccinated intranasally (i.n.) with a temperature sensitive feline infectious peritonitis virus (ts-FIPV) vaccine were protected against an FIP-inducing challenge. Seventeen of 20 vaccinated cats (85%) survived a rigorous virulent FIPV challenge that caused FIP in 12 of 12 non-vaccinated cats (100%), 10 (83%) of which died. Intranasal vaccination stimulated serum IgG and serum and salivary IgA antibody responses (measured by ELISA), FIPV-neutralizing antibody (VN), and a cell-mediated immune (CMI) response as measured by lymphocyte proliferation. The serum antibody response to vaccination was not associated with protection. In fact, the IgG, IgA and VN titres were much higher in control cats than in vaccinated cats following challenge suggesting an immune-mediated pathogenesis. In contrast, stimulation of a mucosal IgA response to vaccination was related to protection. The in vitro proliferation of peripheral blood lymphocytes in response to virulent FIPV was observed in vaccinated cats, in vaccinated and challenged cats but not in non-vaccinated challenged cats.