Glucocorticoid treatment reduces T-bet and pSTAT1 expression in mononuclear cells from relapsing remitting multiple sclerosis patients
Glucocorticoid treatment reduces T-bet and pSTAT1 expression in mononuclear cells from relapsing remitting multiple sclerosis patients
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DOI：
10.1016/j.clim.2007.05.011
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发表时间：
2007-09-01
影响因子：
8.6
通讯作者：
Batocchi, Anna Paola
中科院分区：
文献类型：
--
作者：
Frisullo, Giovanni;Nociti, Viviana;Batocchi, Anna Paola
大剂量糖皮质激素(GC)治疗对复发性多发性硬化(MS)患者的功能恢复有短期的有利作用，但其确切的作用机制尚不清楚。我们发现大剂量静脉注射GC显著降低复发RRMS患者外周血中T-bet和pSTAT1的表达。PSTAT1和T-bet的降低与PBMCs产生的干扰素-γ的减少有关。GC治疗后，AV阳性的CD4+和CD8+T细胞显著增加，Annexin V阳性单核细胞的百分比没有任何变化。通过体外分析，复发的患者，无论是在GC治疗之前还是之后，显示出比缓解期患者和对照组更高比例的凋亡淋巴细胞。我们的研究表明，GCs可以调节T-bet和STAT1的表达，而干扰素-γ信号的抑制有助于GCs在治疗MS患者复发时的抗炎作用。(C)2007 Elsevier Inc.保留所有权利。
High dose glucocorticoid (GC) treatment has been demonstrated to have a short-term beneficial effect on functional recovery in relapsing multiple sclerosis (MS) patients but the exact mechanism of action of GCs in MS is unclear.We found that high dose intravenous GCs strongly reduced T-bet and pSTAT1 expression in CD4+, CD8+, CD14+ circulating cells in RRMS patients in relapse. pSTAT1 and T-bet reduction was associated with the decline of IFN gamma production by PBMCs. A significant increase of AV-positive CD4+ and CD8+ Tcells was detectable after GC treatment without any variation in the percentage of annexin V-positive monocytes. By in vitro analysis, patients during relapse, either before or after GC treatment, exhibited a tower proportion of apoptotic lymphocytes than remitting patients and controls. Our study suggests that GCs can modulate T-bet and STAT1 expression and that IFN gamma signalling inhibition contributes to anti-inflammatory action of GCs in the treatment of relapses of MS patients. (c) 2007 Elsevier Inc. All rights reserved.