A morphometric study of the effects of ethanol consumption on lactating mammary glands of rats.
A morphometric study of the effects of ethanol consumption on lactating mammary glands of rats.
复制标题
乙醇消耗对大鼠哺乳期乳腺影响的形态测量研究。
DOI：
10.1111/j.1530-0277.1989.tb00313.x
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
SeeligJr,LL
中科院分区：
文献类型：
--
作者：
Steven,WM;Bulloch,B;SeeligJr,LL
形态计量学程序被用来定量的变化引起的乙醇在组织成分的大鼠乳腺。大鼠成对喂食为妊娠或哺乳动物配制的含乙醇或等热量对照液体饲料，或维持常规实验室饲料。从妊娠第1天至哺乳第2天或第10天，对短期动物饲喂乙醇或对照饲料。长期饲养动物在交配前配对饲喂乙醇或对照饲料25天，然后在妊娠至哺乳期第2天或第10天。用点计数法测定乳腺腺泡上皮、管腔和结缔组织的体积分数。在饲料喂养的动物中，肺泡上皮细胞的百分比从妊娠晚期到哺乳期保持不变，而结缔组织的数量减少，肺泡腔的数量增加。这表明该程序检测乳腺增生期间组织体积分数变化的灵敏度。在短期乙醇或配对喂养动物中，未发现任何组织成分与正常对照相比发生变化。在哺乳期第2天，与配对喂养和饲料喂养的对照动物相比，长期乙醇处理动物的肺泡上皮细胞百分比显著降低，总结缔组织百分比显著增加。然而，到哺乳期第10天，与对照动物相比，长期乙醇喂养动物的任何组织成分均未发生变化。这些结果表明，乙醇消费可以改变乳腺结构在哺乳期的早期阶段，即使在足够的膳食蛋白质水平保持。
Morphometric procedures were used to quantitate changes induced by ethanol in tissue components of rat mammary gland. Rats were pair‐fed ethanol‐containing or isocaloric control liquid diets formulated for pregnant or lactating animals, or maintained on regular laboratory chow. Short term animals were pair‐fed ethanol or control diets from Day 1 of pregnancy through lactation Days 2 or 10. Long term animals were pair‐fed ethanol or control diets for 25 days prior to mating, and then through pregnancy to lactation Days 2 or 10. Point counting was used to determine the volume fractions (vf) of alveolar epithelium, lumen, and connective tissue in the mammary glands. In chow‐fed animals the percentage of alveolar epithelium remained constant from late pregnancy through lactation, while the amount of connective tissue decreased and that of alveolar lumen increased. This indicates the sensitivity of this procedure to detect changes in tissue volume fractions during mammary proliferation. No changes from the normal controls were found in any tissue component in short term ethanol or pair‐fed animals. At lactation Day 2, long term ethanol‐treated animals demonstrated a significant decrease in the percentage of alveolar epithelium and a significant increase in the percentage of total connective tissue as compared to pair‐fed and chow‐fed control animals. However, by Day 10 of lactation, no changes were found in any of the tissue components in long‐term ethanol‐fed versus control animals. These results indicate that ethanol consumption can alter mammary gland structure during the early stages of lactation, even when adequate levels of dietary protein are maintained.