CD147 promotes progression of head and neck squamous cell carcinoma via NF-kappa B signaling
CD147 promotes progression of head and neck squamous cell carcinoma via NF-kappa B signaling
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CD147 通过 NF-κ B 信号促进头颈鳞状细胞癌的进展
DOI：
10.1111/jcmm.13996
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发表时间：
2019
影响因子：
5.3
通讯作者：
Yan Ming
中科院分区：
文献类型：
--
作者：
Yu Binbin;Zhang Yan;Wu Kailiu;Wang Lizhen;Jiang Yingying;Chen Wantao;Yan Ming
CD 147/basigin（BSG）在多种肿瘤中表达上调，我们前期的研究发现CD 147/BSG在头颈部鳞状细胞癌（HNSCC）干细胞中高表达，但其在HNSCC中的作用及其机制尚不清楚。在这项研究中，我们研究了CD 147在HNSCC进展中的作用。采用真实的-time PCR、western blot和免疫组化方法检测189例HNSCC组织中CD 147的表达，并与正常组织进行比较。此外，我们还通过增殖、集落形成、细胞周期和凋亡、迁移和侵袭以及伤口愈合试验来确定CD 147在HNSCC中的生物学作用。然后，进行异种移植模型以评估⑶ 147在HNSCC中的促肿瘤和促转移作用。结果表明，上调的CD 147表达与HNSCC的侵袭性临床病理特征相关。此外，CD 147在体外促进HNSCC细胞的增殖、迁移和减少凋亡表型，以及在体内促进肿瘤的发生和发展。此外，我们证明了CD 147通过核因子κ B信号传导促进HNSCC进展。因此，我们得出结论，CD 147促进了HNSCC的肿瘤进展，可能是HNSCC的潜在预后和治疗生物标志物。
CD147/basigin (BSG) is highly upregulated in many types of cancer, our previous study has found that CD147/BSG is highly expressed in head and neck squamous cell carcinoma (HNSCC) stem cells, but its role in HNSCC and the underlying mechanism is still unknown. In this study, we investigated the role of CD147 in the progression of HNSCC. Real‐time PCR, western blot and immunohistochemistry were used to detect the expression of CD147 in total 189 HNSCC tissues in compared with normal tissues. In addition, we used proliferation, colony formation, cell cycle and apoptosis, migration and invasion as well as wound‐healing assay to determine the biological roles of CD147 in HNSCC. Then, a xenograft model was performed to evaluate tumor‐promoting and metastasis‐promoting role of CD147 in HNSCC. The results showed that upregulated CD147 expression was associated with aggressive clinicopathologic features in HNSCC. In addition, CD147 promoted proliferation, migration and reduced the apoptosis phenotype of HNSCC cells in vitro as well as tumor initiation and progression in vivo. Furthermore, we demonstrated that CD147 promoted HNSCC progression through nuclear factor kappa B signaling. Therefore, we concluded that CD147 promoted tumor progression in HNSCC and might be a potential prognostic and treatment biomarker for HNSCC.