The performance of a CCD digital autoradiography imaging system
The performance of a CCD digital autoradiography imaging system
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DOI：
10.1088/0031-9155/45/7/322
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发表时间：
2000-07-01
影响因子：
3.5
通讯作者：
Wells, K
中科院分区：
文献类型：
--
作者：
Ott, R;MacDonald, J;Wells, K
放射自显影是一种广泛使用的技术，用于对生物样品中的痕量放射性进行成像，通常使用照相胶片。该方法产生具有高空间分辨率的图像，但是其遭受非常低的灵敏度和差的动态范围。灵敏度和线性度大大提高的数字放射自显影系统在商业上是可用的，但空间分辨率通常远低于使用他实现的。我们在这里报告的设计，建设和表征的一种新型的数字放射自显影系统的基础上科学级电荷耦合器件（CCD）。放射自显影中常用的放射性核素的X射线和β发射的图像显示，该系统可以进行高速定量成像，空间分辨率接近30 μ m。使用逐帧采集方法的动态范围被示出为至少三个数量级。绝对检测效率可与目前可用的最佳数字系统相媲美。组织样本中（125）I和C-14放射性同位素分布的CCD图像上级等效胶片图像，并且在1-10%的时间内获得。
Autoradiography is a widely used technique for imaging trace quantities of radioactivity within biological samples, conventionally using photographic film. This method produces images with high spatial resolution, but it suffers from very low sensitivity and poor dynamic range. Digital autoradiography systems with greatly improved sensitivity and linearity are commercially available, but the spatial resolution is usually much less than that achieved using him. We report here the design, construction and characterization of a novel digital autoradiography system based on scientific-grade charged coupled devices (CCDs). Images of x-ray and beta emissions from radionuclides commonly used in autoradiography show that the system can perform highspeed quantitative imaging with a spatial resolution of similar to 30 mu m. Using a frame by frame acquisition method the dynamic range is shown to be at least three orders of magnitude. The absolute detection efficiency is comparable to the best of the currently available digital systems. CCD images of(125)I and C-14 radioisotope distributions in tissue samples are superior to the equivalent film images and have been acquired in 1-10% of the time.