Barbiturate-induced inhibition of rat brain histamine turnover.
Barbiturate-induced inhibition of rat brain histamine turnover.
复制标题
巴比妥诱导的大鼠脑组胺周转抑制。
DOI：
10.1016/0006-2952(87)90656-3
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发表时间：
1987
影响因子：
5.8
通讯作者：
Hough,LB
中科院分区：
文献类型：
--
作者：
Hough,LB
将雄性SpragueDawley大鼠（225-300 g，TaconicFarms，日耳曼敦，纽约州）维持在12小时的光-暗循环中，并接受以下四种组合的两种单独的ip注射之一：盐水/盐水、盐酸帕吉林（75 kg盐水）/盐水、盐水/钠和妥巴比妥（50 mg）。可注射制剂。雅培公司），br p&g $ nd/戊巴比妥（相同剂量）。4小时后（进入光照周期4小时），在室温下将动物斩首，并迅速取出其大脑并在冰上解剖。在Polytron匀浆器中，将组织在5-10体积的冰冷去离子水中匀浆15次，然后用等份的0.1 N磷酸钠缓冲液（pH 7.9）（用于测量HA）或等份的0.8 N高氯酸（用于测量t-MH）稀释。一项单独的实验证实，戊巴比妥溶剂（含40%丙二醇和10%乙醇的盐水）对全脑HA或r-MH水平或HA周转率无影响。戊巴比妥给药动物在注射后4小时间隔的前2.5小时睡眠。先前的研究[9，10]表明帕吉林诱导的t-MH蓄积速率在前4小时内是恒定的，因此选择了该间隔。
MethodsMale SpragueDawley rats (225-300 g, Taconic Farms, Germantown, NY) were maintained in 12-hr light-dark cycles, and received one of the following four combinations of two separate ip injections: saline/saline, pargyline hydrochloride (75 kg sal&g)/saline, saline/sodium {&tobarbital (SO mea. iniectable formulation. Abbott Laboratories), br p&g $ nd/pentobarbital(same doses). Four hours later (4 hr into the light cycle), the animals were decapitated at room temperature, and their brains were removed rapidly and dissected on ice. Tissues were homogenized in 5-10 vol. of ice-cold deionized water for 15 set in a Polytron homogenizer, and then diluted with an equal part of either 0.1 N sodum phosphate buffer (pH 7.9)(to measure HA) or an equal part of 0.8 N perchloric acid (to measure t-MH). A separate experiment verified that the pentobarbital vehicle (saline containing 40% propylene glycol and 10% ethanol) had no effect on whole brain HA or r-MH levels, or on HA turnover rates. Pentobarbitaltreated animals slept for approximately the first 2.5 hr of the 4-hr post-injection interval. Previous studies [9, 10] have shown that the rate of pargyline-induced accumulation oft-MH is constant for the first 4 hr, hence the choice of this interval.