Evaluation of electrospun PCL/gelatin nanofibrous scaffold for wound healing and layered dermal reconstitution
Evaluation of electrospun PCL/gelatin nanofibrous scaffold for wound healing and layered dermal reconstitution
复制标题
DOI：
10.1016/j.actbio.2007.01.002
复制
发表时间：
2007-05-01
影响因子：
9.7
通讯作者：
Lim, C. T.
中科院分区：
文献类型：
--
作者：
Chong, E. J.;Phan, T. T.;Lim, C. T.
目前组织工程皮肤替代物的设计要求是可生物降解的支架，成纤维细胞可以通过该支架迁移和繁殖。这种人造"真皮层“需要粘附到伤口上并与伤口整合，这对于目前可用的人造真皮类似物来说并不总是成功的。这些人造真皮类似物的高成本也使得它们的应用对外科医生和患者都是禁止的。我们提出了一种具有成本效益的复合材料，由直接静电纺丝到聚氨酯敷料（Tegaderm-TM，3 M Medical）上的纳米纤维支架组成-我们称之为Tegaderm-TM（TG-NF）结构-用于皮肤伤口愈合。在纳米纤维支架的两侧进行细胞培养，并测试成纤维细胞粘附和增殖。希望这些研究将产生成纤维细胞填充的三维真皮模拟物，其对于分层应用是可行的，以在再上皮化之前建立真皮厚度。本研究中获得的结果表明，TG-NF构建体和双侧成纤维细胞填充的血管内皮细胞构建体均实现了显著的细胞粘附、生长和增殖。这是纳米纤维构建体成功的第一步，将其自身建立为自体成纤维细胞群体的合适三维支架，并通过分层应用为治疗皮肤伤口提供了巨大潜力。(C)2007 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
The current design requirement for a tissue engineering skin substitute is that of a biodegradable scaffold through which fibroblasts can migrate and populate. This artificial '' dermal layer '' needs to adhere to and integrate with the wound, which is not always successful for the current artificial dermal analogues available. The high cost of these artificial dermal analogues also makes their application prohibitive both to surgeons and patients. We propose a cost-effective composite consisting of a nanofibrous scaffold directly electrospun onto a polyurethane dressing (Tegaderm((TM)), 3M Medical) - which we call the Tegaderm-nanofiber (TG-NF) construct - for dermal wound healing. Cell culture is performed on both sides of the nanofibrous scaffold and tested for fibroblast adhesion and proliferation. It is hoped that these studies will result in a fibroblast-populated three-dimensional dermal analogue that is feasible for layered applications to build up thickness of dermis prior to re-epithelialization. Results obtained in this study suggest that both the TG-NF construct and dual-sided fibroblast-populated nanofiber construct achieved significant cell adhesion, growth and proliferation. This is a successful first step for the nanofiber construct in establishing itself as a suitable three-dimensional scaffold for autogenous fibroblast populations, and providing great potential in the treatment of dermal wounds through layered application. (C) 2007 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.