Antifungal activity, kinetics and molecular mechanism of action of garlic oil against Candida albicans.
Antifungal activity, kinetics and molecular mechanism of action of garlic oil against Candida albicans.
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大蒜油对白色念珠菌的抗真菌活性、动力学和分子机制
DOI：
10.1038/srep22805
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发表时间：
2016-03-07
影响因子：
4.6
通讯作者：
Zhang LX
中科院分区：
文献类型：
--
作者：
Li WR;Shi QS;Dai HQ;Liang Q;Xie XB;Huang XM;Zhao GZ;Zhang LX
采用多种方法研究了大蒜油对白色念珠菌的抑菌活性、动力学及分子机制。采用中毒食品法测定大蒜油的最低抑菌浓度为0.35 μg/mL。透射电镜观察表明，大蒜油能穿透细胞细胞膜。白色念珠菌以及细胞器的膜，如线粒体，导致细胞器的破坏和最终细胞死亡。RNA测序分析显示，大蒜油诱导了包括氧化还原过程、发病机制和细胞对药物和饥饿反应相关基因在内的关键基因的差异表达。此外，差异表达基因主要聚集在19个KEGG通路上，代表了重要的细胞过程，如氧化磷酸化、剪接体、细胞周期和内质网蛋白质加工。此外，通过二维荧光凝胶电泳（2D-DIGE）分析选择的4个上调蛋白，通过质谱高概率鉴定为细胞质腺苷酸激酶、丙酮酸脱羧酶、己糖激酶和热休克蛋白。这提示白色ac对大蒜油处理有应激反应。另一方面，大量蛋白质下调，导致c的正常代谢和生理功能受到严重破坏。白色的。
The antifungal activity, kinetics and molecular mechanism of action of garlic oil againstCandida albicanswere investigated in this study using multiple methods. Using the poisoned food technique, we determined that the minimum inhibitory concentration of garlic oil was 0.35 μg/mL. Observation by transmission electron microscopy indicated that garlic oil could penetrate the cellular membrane ofC. albicansas well as the membranes of organelles such as the mitochondria, resulting in organelle destruction and ultimately cell death. RNA sequencing analysis showed that garlic oil induced differential expression of critical genes including those involved in oxidation-reduction processes, pathogenesis and cellular response to drugs and starvation. Moreover, the differentially expressed genes were mainly clustered in 19 KEGG pathways, representing vital cellular processes such as oxidative phosphorylation, the spliceosome, the cell cycle and protein processing in the endoplasmic reticulum. In addition, four upregulated proteins selected after two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE) analysis were identified with high probability by mass spectrometry as putative cytoplasmic adenylate kinase, pyruvate decarboxylase, hexokinase and heat shock proteins. This is suggestive of aC. albicansstress responses to garlic oil treatment. On the other hand, a large number of proteins were downregulated, leading to significant disruption of the normal metabolism and physical functions ofC. albicans.