Effects of exposure to nanoparticle-rich diesel exhaust on adrenocortical function in adult male mice
Effects of exposure to nanoparticle-rich diesel exhaust on adrenocortical function in adult male mice
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DOI：
10.1016/j.toxlet.2012.01.004
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发表时间：
2012-03-25
影响因子：
3.5
通讯作者：
Taya, Kazuyoshi
中科院分区：
文献类型：
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作者：
Li, ChunMei;Li, Xuezheng;Taya, Kazuyoshi
为了研究富含纳米颗粒的柴油机尾气（NR-DE）对肾上腺皮质功能的影响，将七周大的雄性小鼠分为四组，并分别暴露于低浓度（41.73 μ g/m(3)、8.21 x 10(5) 颗粒/cm(3)）和高浓度（152.01 μ g/m(3)、1.80 x 10(6) 颗粒/cm(3)）的完整NR-DE。浓度、过滤柴油废气 (F-DE) 或清洁空气，持续 8 周（每天 5 小时，每周 5 天）。暴露8周后，在戊巴比妥麻醉下对动物实施安乐死，并采集血样检测血清孕酮和皮质酮。此外，切除肾上腺，并在不存在或存在大鼠促肾上腺皮质激素(ACTH)(10(-15)至10(-10)M)的情况下培养肾上腺细胞4小时。各组之间的体重、绝对和相对肾上腺重量没有显着差异。皮质酮和孕酮的血清浓度没有显着变化。暴露于 lovv 浓度 NR-DE 和清洁空气的小鼠中，给予 ACTH 会导致皮质酮和孕酮释放呈剂量依赖性增加。此外，与对照小鼠相比，暴露于低浓度NR-DE基础液并施用ACTH（皮质酮为10（-15）至10（-11）M；黄体酮为10（-14）至10（-11）M）的小鼠肾上腺细胞中皮质酮和孕酮浓度显着增加。相反，与对照小鼠相比，暴露于高浓度NR-DE或F-DE基础液并给予ACTH（皮质酮为10（-12）至10（-10）M；黄体酮为10（-15）至10（-10）M）的小鼠的皮质酮和孕酮浓度显着降低。这些结果表明，暴露于 NR-DE 或 F-DE 可能会破坏成年雄性小鼠的肾上腺皮质功能。 (C) 2012 Elsevier Ireland Ltd. 保留所有权利。
To investigate the effects of nanoparticle-rich diesel exhaust (NR-DE) on adrenocortical function, seven-week-old male mice were divided into four groups and exposed to either whole NR-DE at low (41.73 mu g/m(3), 8.21 x 10(5) particles/cm(3)), high (152.01 mu g/m(3), 1.80 x 10(6) particles/cm(3)) concentrations, filtered diesel exhaust (F-DE) or clean air for 8 weeks (5h/day, 5 days/week). After 8 weeks of exposure, the animals were euthanized under pentobarbital anesthesia and the blood samples were collected to detect serum progesterone and corticosterone. In addition, adrenal glands were excised, and adrenal cells were cultured in the absence or presence of rat adrenocorticotropic hormone (ACTH) (10(-15) to 10(-10) M) for 4 h. There were no significant differences in the body weight, absolute and relative adrenal gland weight among the groups. Serum concentration of corticosterone and progesterone was not changed significantly. Administration of ACTH resulted in a dose-dependent increase in corticosterone and progesterone release in mice-exposed to lovv-concentration NR-DE and clean air. Moreover, corticosterone and progesterone concentrations in adrenal cells increased significantly in mice-exposed to low-concentration NR-DE basal and administrated with ACTH (10(-15) to 10(-11) M for corticosterone; 10(-14) to 10(-11) M for progesterone) compared with the control mice. In contrast, the concentration of corticosterone and progesterone decreased significantly in mice-exposed to high-concentration NR-DE or F-DE basal and administrated with ACTH (10(-12) to 10(-10) M for corticosterone; 10(-15) to 10(-10) M for progesterone) compared with the control mice. These results suggest that exposure to NR-DE or F-DE may disrupt adrenocortical function in adult male mice. (C) 2012 Elsevier Ireland Ltd. All rights reserved.