Polymyxin-based enzyme-linked immunosorbent assay for the detection of Escherichia coli O111 and O26
Polymyxin-based enzyme-linked immunosorbent assay for the detection of Escherichia coli O111 and O26
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DOI：
10.1016/j.mimet.2005.09.004
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发表时间：
2006-06-01
影响因子：
2.2
通讯作者：
Popela, M
中科院分区：
文献类型：
--
作者：
Blais, BW;Bosley, J;Popela, M
开发了基于多粘菌素的酶联免疫吸附测定（多粘菌素-ELISA）系统，用于检测富集后碎牛肉中的大肠杆菌 O111 和 O26。固定在微量滴定板孔中的多粘菌素作为脂多糖（LPS）抗原的高亲和力吸附剂，使用市售的抗大肠杆菌进行免疫酶检测。大肠杆菌 O111 或抗大肠杆菌。大肠杆菌 O26 抗血清。多粘菌素-ELISA 灵敏地检测带有 O111 和 O26 LPS 抗原的大肠杆菌菌株，区分这些目标菌株和一组各种非目标革兰氏阴性和革兰氏阳性细菌。在含有新生霉素的改良胰蛋白酶大豆肉汤 (TSB) 或补充有头孢克肟和万古霉素的血清型特异性培养基 TSB (大肠杆菌 O111) 以及含有亚碲酸钾的相同培养基 (大肠杆菌 O26) 中富集后，可检测接种到碎牛肉中的大肠杆菌 O111 和 O26 菌株。多粘菌素-ELISA 有望成为碎牛肉富集培养物中大肠杆菌 O111 和 O26 的快速、简单且廉价的筛选工具 (c) 2005 Elsevier B.V. 保留所有权利。
Polymyxin-based enzyme-linked inununosorbent assay (polymyxin-ELISA) systems were developed for the detection of Escherichia coli O111 and O26 in ground beef after enrichment. Polymyxin immobilized in the wells of a microtiter plate served as a high affinity adsorbent for lipopolysaccharide (LPS) antigens, which were detected immunoenzymatically using commercially available anti-E. coli O111 or anti-E. coli O26 antisera. The polymyxin-ELISA sensitively detected E. coli strains bearing the O111 and O26 LPS antigens, discriminating between these target strains and a panel of various non-target Gram negative and Gram positive bacteria. The detection of E. coli O111 and O26 strains inoculated into ground beef was achieved after enrichment in either modified trypticase soy broth (TSB) with novobiocin, or the serotype-specific medium TSB supplemented with cefixime and vancomycin (E. coli O111), and the same medium containing potassium tellurite (E. coli O26). The polymyxin-ELISA shows promise as a rapid, simple and inexpensive screening tool for E. coli O111 and O26 in enrichment cultures of ground beef (c) 2005 Elsevier B.V. All rights reserved.