Arabidopsis thaliana, an experimental host for tomato yellow leaf curl disease-associated begomoviruses by agroinoculation and whitefly transmission
Arabidopsis thaliana, an experimental host for tomato yellow leaf curl disease-associated begomoviruses by agroinoculation and whitefly transmission
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DOI：
10.1111/ppa.12270
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发表时间：
2015-04-01
期刊：
影响因子：
2.7
通讯作者：
Moriones, E.
中科院分区：
文献类型：
--
作者：
Canizares, M. C.;Rosas-Diaz, T.;Moriones, E.
番茄黄化曲叶病是世界范围内影响番茄作物的最具破坏性的病毒病之一。该疾病由几种菜豆病毒（菜豆病毒属，双生病毒科）引起，例如番茄黄化曲叶病毒（TYLCV），其在自然界中由粉虱载体Bemisiatabaci传播。有效控制这种媒介传播的疾病需要彻底了解植物-病毒-媒介三重相互作用。使用拟南芥作为实验宿主的可能性将提供机会，使用各种各样的遗传资源和工具，以了解在农学上重要的主机是不可行的相互作用。在这项研究中，证明了TYLCV的两个菌株（以色列，IL和温和，Mld）的分离物可以复制和系统感染拟南芥生态型哥伦比亚植物无论是通过农杆菌介导的接种或通过天然载体Bemisiatabaci。该病毒也可以通过B.tabaci从拟南芥感染的植物中获得，并传播给拟南芥或番茄植物。因此，拟南芥是TYLCV-昆虫载体-植物宿主互作研究的合适宿主。有趣的是，一种相关的菜豆病毒，番茄黄化曲叶撒丁岛病毒（TYLCSV-ES）的西班牙（ES）株的分离物不能有效地感染这种生态型的拟南芥。使用TYLCV-Mld和TYLCSV-ES之间的感染性嵌合病毒克隆，鉴定了参与拟南芥有效感染的候选病毒因子。
Tomato yellow leaf curl disease is one of the most devastating viral diseases affecting tomato crops worldwide. This disease is caused by several begomoviruses (genus Begomovirus, family Geminiviridae), such as Tomato yellow leaf curl virus (TYLCV), that are transmitted in nature by the whitefly vector Bemisiatabaci. An efficient control of this vector-transmitted disease requires a thorough knowledge of the plant-virus-vector triple interaction. The possibility of using Arabidopsisthaliana as an experimental host would provide the opportunity to use a wide variety of genetic resources and tools to understand interactions that are not feasible in agronomically important hosts. In this study, it is demonstrated that isolates of two strains (Israel, IL and Mild, Mld) of TYLCV can replicate and systemically infect A.thaliana ecotype Columbia plants either by Agrobacteriumtumefaciens-mediated inoculation or through the natural vector Bemisiatabaci. The virus can also be acquired from A.thaliana-infected plants by B.tabaci and transmitted to either A.thaliana or tomato plants. Therefore, A.thaliana is a suitable host for TYLCV-insect vector-plant host interaction studies. Interestingly, an isolate of the Spain (ES) strain of a related begomovirus, Tomato yellow leaf curl Sardinia virus (TYLCSV-ES), is unable to infect this ecotype of A.thaliana efficiently. Using infectious chimeric viral clones between TYLCV-Mld and TYLCSV-ES, candidate viral factors involved in an efficient infection of A.thaliana were identified.