Comprehensive identification of odorant-binding protein genes in the seed fly, Delia platura (Diptera: Anthomyiidae)
Comprehensive identification of odorant-binding protein genes in the seed fly, Delia platura (Diptera: Anthomyiidae)
复制标题
种蝇 Delia platura（双翅目：花蝇科）气味结合蛋白基因的综合鉴定
DOI：
10.1007/s13355-015-0353-8
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发表时间：
2015
影响因子：
1.3
通讯作者：
Matsuo T.
中科院分区：
文献类型：
--
作者：
Ohta S;Seto Y;Tamura K;Ishikawa Y;Matsuo T.
昆虫气味结合蛋白（OBP）在昆虫对信息素和寄主植物气味等化学信号的感知中发挥重要作用。比较基因组分析表明，OBP基因经历了出生和死亡的过程，通过进化的六足类，导致在远亲物种之间的OBP剧目分歧。另一方面，密切相关的物种之间的比较，特别是那些具有不同程度的主机专业化的物种，是有限的。本研究通过对触角和足的转录组分析，从扁腹迪莉娅（Delia platura（Meigen））中鉴定出20个新的OBP基因。的剧目OBP基因进行了比较，一个密切相关的物种，迪利亚antiqua（梅根）。虽然D。柏拉图是一个多面手，而D. antiquais是宿主利用方面的专家，这些OBP的表达谱在两个物种之间几乎相同。进化分析表明，至少有14个OBP已经纯化选择，这表明它们在两个物种中具有相似的功能。这些结果表明，在这些物种的OBP剧目是相对独立的主机专业化。
Insect odorant-binding proteins (OBPs) function in the perception of chemical cues such as pheromones and host-plant odors. Comparative genomic analysis has shown that OBP genes underwent the birth-and-death process through the evolution of Hexapoda, resulting in diverged OBP repertoires between distantly related species. On the other hand, comparisons between closely related species, particularly those species with different degrees of host specialization, are limited. In this study, 20 novel OBP genes were identified fromDelia platura(Meigen) by transcriptome analysis in the antennae and legs. The repertoire of OBP genes was compared with that of a closely related species,Delia antiqua(Meigen). AlthoughD. platurais a generalist whileD. antiquais a specialist in regard to their host utilization, expression profiles of these OBPs were almost identical between the two species. Evolutionary analysis showed that at least 14 OBPs have been under purifying selection, suggesting that they have a similar function in both species. These results suggest that the repertoire of OBPs was relatively independent from host specialization in these species.