Expression of Heparanase Is Associated with Breed-Specific Morphological Characters of Placental Folded Bilayer Between Yorkshire and Meishan Pigs
Expression of Heparanase Is Associated with Breed-Specific Morphological Characters of Placental Folded Bilayer Between Yorkshire and Meishan Pigs
复制标题
乙酰肝素酶的表达与约克夏猪和梅山猪胎盘折叠双层的品种特异性形态特征相关
DOI：
10.1095/biolreprod.113.114181
复制
发表时间：
2014-03-01
影响因子：
3.6
通讯作者：
Yu, Mei
中科院分区：
文献类型：
--
作者：
Hong, Linjun;Hou, Chunyan;Yu, Mei
猪具有非侵袭性上皮绒毛膜胎盘，滋养层-子宫内膜上皮双层的发育可能影响胎盘功能。本研究比较了妊娠26、50和95天约克郡猪和梅山猪母胎界面的形态结构、Ki 67染色检测细胞增殖状态、乙酰肝素酶（HPSE）基因和蛋白表达的变化。组织形态计量学结果表明，梅山猪妊娠晚期胎盘皱襞、子宫内膜间质和胎盘间质的宽度均小于约克夏猪，但折叠双层的复杂程度和细胞增殖能力均大于约克夏猪。HPSE mRNA和蛋白在妊娠早期和中期母胎界面的位置和表达水平在两个品种之间是相似的。然而，在妊娠晚期，mRNA和蛋白质水平较高的梅山胎盘。此外，梅山猪妊娠晚期所有滋养层细胞均表达HPSE mRNA，且HPSE蛋白定位于滋养层细胞和腔上皮细胞，而约克郡猪仅在皱襞底部和侧部的滋养层细胞表达HPSE mRNA和蛋白。结果提示，梅山猪胎盘结构的变化主要是由于胎盘内折叠双层结构的复杂性增加，从而增加了交换表面积，HPSE可能促进了折叠双层结构的形成。
The pig has a noninvasive epitheliochorial placenta, and trophoblast-endometrial epithelial bilayer development could impact on placental function. This work compared the morphological structures, the cell proliferation status as assessed by Ki67 staining, as well as the location and gene and protein expression of heparanase (HPSE) at the maternal-fetal interface between Yorkshire and Meishan pigs on Days 26, 50, and 95 of gestation. Histomorphometry showed that the widths of placental folds, endometrial stroma, and placental stroma in Meishan pigs were smaller than those in Yorkshire pigs during late gestation, while the complexity and the cell proliferation ability of the folded bilayer were greater in Meishan pigs in this period. The location and expression levels of HPSE mRNA and protein at the maternal-fetal interface were similar between the two breeds during early and midgestation. However, during late gestation, the mRNA and protein levels were higher in Meishan placentae. In addition, the HPSE mRNA was expressed by all the trophoblast cells, and the protein was located both at trophoblast and luminal epithelium cells in Meishan pigs during late gestation, while in Yorkshire pigs, the HPSE mRNA and protein were only identified in trophoblast cells located at the bottom and side of folds. The findings suggest that Meishan pigs may rely more upon the increase in the complexity of the folded bilayer within a reduced placenta to expand the exchange surface area and the HPSE may contribute to the development of the folded bilayer in pigs.