Synthesis of dispersive iron or iron–silver nanoparticles on engineered capsid pVIII of M13 virus with electronegative terminal peptides
Synthesis of dispersive iron or iron–silver nanoparticles on engineered capsid pVIII of M13 virus with electronegative terminal peptides
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DOI：
10.1007/s11051-015-3221-0
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发表时间：
2015-10
影响因子：
2.5
通讯作者：
Shuai Zhang;K. Nakano;Shuliang Zhang;Huimin Yu
中科院分区：
文献类型：
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作者：
Shuai Zhang;K. Nakano;Shuliang Zhang;Huimin Yu
M13是一种被5种衣壳蛋白覆盖的丝状大肠杆菌病毒，其中约2700个拷贝的pVIII位于圆柱形表面，5个拷贝的pIII位于噬菌体颗粒的一端。经过5轮生物筛选，筛选出对铁结合特异性增强的piii工程M13噬菌体，选择piii末端含有ATPTVAMSLSPL肽的噬菌体，以野生M13为对照，介导合成零价（ZV）铁纳米颗粒（NPs）。在还原环境下，合成了均匀分散的直径为5 ~ 10 nm的ZVFeNPs，退火后的形貌为面心立方型。两种噬菌体介导的合成FeNPs无显著差异，表明pVIII衣壳对金属结合的贡献大于pIII衣壳。构建了一个末端含有AAEEEDPAK的新型pviii工程M13，命名为4ED-pVIII-M13，它比野生的aeeedpak多携带一个带负电荷的残基。金属吸附定量分析表明，4ED-pVIII-M13对Ag和Ni离子的结合亲和力分别由原来的21%和6%提高到62%和18%。对铁的结合亲和力保持不变（~ 85%）。以4ED-pVIII-M13为媒介成功合成了zfe - ag双nps。特别是，通过改变还原前fec2和agno3溶液的摩尔浓度，可以方便地控制bi-NPs中的Fe:Ag比。
M13 is a filamentousEscherichia colivirus covered with five types of capsid proteins, in which pVIII with ~2700 copies was around the cylindered surface and pIII with five copies located at one end of the phage particle. The pIII-engineered M13 phages with enhanced binding specificity toward Fe were screened after five rounds of biopanning, and the one containing ATPTVAMSLSPL peptide at pIII-terminus was selected for mediated synthesis of zero valent (ZV) Fe nanoparticles (NPs) with the wild M13 as control. Under a reducing environment, uniformly dispersed ZVFeNPs with diameter of 5–10 nm were both synthesized and the morphologies after annealing were confirmed to be face-centered cubic type. The synthesized FeNPs mediated by the two phages showed no significant difference, revealing that the pVIII capsid did dominant contribution to metal binding in comparison with the pIII. A novel pVIII-engineered M13 containing AAEEEDPAK at terminus, named as 4ED-pVIII-M13, was constructed and it carried one more negatively charged residue than the wild one (AEGDDPAK). Metal adsorption quantification showed that the binding affinity of the 4ED-pVIII-M13 toward Ag and Ni ions improved to 62 and 18 % from original 21 and 6 %, respectively. The binding affinity toward Fe remained constant (~85 %). ZVFe–Ag bi-NPs were successfully synthesized through mediation of 4ED-pVIII-M13. Particularly, the Fe:Ag ratio in the bi-NPs was conveniently controlled through changing the molar concentration of FeCl2and AgNO3solution before reduction.