Hydrogen peroxide scavenging regulates germination ability during wheat (Triticum aestivum L.) seed maturation
Hydrogen peroxide scavenging regulates germination ability during wheat (Triticum aestivum L.) seed maturation
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DOI：
10.4161/psb.3.3.5540
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发表时间：
2008-01-01
影响因子：
2.9
通讯作者：
Iwaya-Inoue, Mari
中科院分区：
文献类型：
--
作者：
Ishibashi, Yushi;Yamamoto, Kouhei;Iwaya-Inoue, Mari
过氧化氢（H2 O2）促进禾本科植物种子萌发，抗坏血酸作为抗氧化剂抑制小麦种子萌发，但在种子成熟过程中清除H2 O2对萌发的作用尚未得到证实。研究了两种典型小麦（Triticum aestivum L.）栽培品种，cvs. Shirogane-Komugi和Norin 61.在种子成熟过程中，白金-小牧的发芽力显著高于Norin 61。在种子成熟过程中，虽然两个品种的H2 O2含量没有差异，但Norin 61种子对阿萨的敏感性高于Shirogane-Komugi种子。两个品种对阿萨的敏感性与其发芽特性密切相关。特别是授粉后28 d（DAP），Norin 61种子对阿萨的敏感性显著提高。在此阶段，两个品种的内源阿萨水平、抗坏血酸过氧化物酶（APX，EC 1.11.1.11）和脱氢抗坏血酸还原酶（达尔，EC 1.8.5.1）活性没有显著差异。然而，过氧化氢酶（CAT，EC 1.11.1.6）活性和CAT mRNA在Norin 61中显著高于Shirogane-Komugi。在35和42 DAPs时，Norin 61对阿萨的敏感性保持较高水平，种子内源阿萨和CAT活性显著高于Shirogane-Komugi。这些结果揭示了小麦种子萌发和抗氧化剂敏感性之间的直接相关性。
Hydrogen peroxide (H2O2) promotes seed germination of cereal plants and ascorbic acid which acts as antioxidant suppresses the germination of wheat seeds, but the role of H2O2 scavenging on germination during seed maturation has not been demonstrated. We investigated relationship of germination, ascorbate, H2O2 scavenging enzymes and sensitivity to ascorbic acid (AsA) maturing seeds of two typical wheat (Triticum aestivum L.) cultivars, cvs. Shirogane-Komugi and Norin61. Shirogane-Komugi had marked high germination ability than Norin61 during seed maturation. Although the H2O2 content had no difference in the two cultivars, sensitivity to AsA of Norin61 seeds was higher than that of Shirogane-Komugi seeds during seed maturation. The sensitivity to AsA closely correlated with germination characteristic in the two cultivars. Especially, at 28 days after pollination (DAP), sensitivity to AsA in Norin61 seeds was remarkably high. At that stage, no significant differences were observed in endogenous AsA level, ascorbate peroxidase (APX, EC 1.11.1.11) and dehydroascorbate reductase (DHAR, EC 1.8.5.1) activities in the two cultivars. However, catalase (CAT, EC 1.11.1.6) activity and CAT mRNA in Norin61 were remarkably higher than in Shirogane-Komugi. Sensitivity to AsA at 35 and 42 DAPs kept high levels in Norin61, and endogenous AsA and CAT activity in the seeds were significantly higher than in Shirogane-Komugi. These results revealed a direct correlation between germination and antioxidant sensitivity during the developmental stages of wheat seeds.