Exploring T cell reactivity to gliadin in young children with newly diagnosed celiac disease.
Exploring T cell reactivity to gliadin in young children with newly diagnosed celiac disease.
复制标题
探索新诊断乳糜泻幼儿的 T 细胞对麦醇溶蛋白的反应性。
DOI：
10.1155/2014/927190
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发表时间：
2014
影响因子：
4
通讯作者：
Michels,AaronW
中科院分区：
文献类型：
--
作者：
Liu,Edwin;McDaniel,Kristen;Case,Stephanie;Yu,Liping;Gerhartz,Bernd;Ostermann,Nils;Fankhauser,Gabriela;Hungerford,Valerie;Zou,Chao;Luyten,Marcel;Seidl,KatherineJ;Michels,AaronW
Class II major histocompatibility molecules confer disease risk in Celiac disease (CD) by presenting gliadin peptides to CD4 T cells in the small intestine. Deamidation of gliadin peptides by tissue transglutaminase creates immunogenic peptides presented by HLA‐DQ2 and DQ8 molecules to activate proinflammatory CD4 T cells. Detecting gliadin specific T cell responses from the peripheral blood has been challenging due to low circulating frequencies and heterogeneity in response to gliadin epitopes. We investigated the peripheral T cell responses to alpha and gamma gliadin epitopes in young children with newly diagnosed and untreated CD. Using peptide/MHC recombinant protein constructs, we are able to robustly stimulate CD4 T cell clones previously derived from intestinal biopsies of CD patients. These recombinant proteins and a panel ofα‐ andγ‐gliadin peptides were used to assess T cell responses from the peripheral blood. Proliferation assays using peripheral blood mononuclear cells revealed more CD4 T cell responses toα‐gliadin thanγ‐gliadin peptides with a single deamidatedα‐gliadin peptide able to identify 60% of CD children. We conclude that it is possible to detect T cell responses without a gluten challenge or in vitro stimulus other than antigen, when measuring proliferative responses.
