Genome-scale profiling of histone H3.3 replacement patterns
Genome-scale profiling of histone H3.3 replacement patterns
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DOI：
10.1038/ng1637
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发表时间：
2005-10-01
期刊：
影响因子：
30.8
通讯作者：
Henikoff, S
中科院分区：
文献类型：
--
作者：
Mito, Y;Henikoff, JG;Henikoff, S
多细胞生物的组蛋白主要在DNA复制过程中组装成染色质。当染色质组装发生在其他时间，组蛋白H3.3变体取代典型的H3。在这里，我们介绍了一种新的策略，分析H3.3替代的基础上的表观遗传模式，使用微阵列覆盖约三分之一的果蝇基因组在100 bp的分辨率。我们确定了H3.3取代活性基因和转座子的模式。H3.3置换显著发生在整个基因组中丰富的RNA聚合酶II和甲基化H3 Lys4的位点，并在剂量补偿的男性X染色体上增强。活性基因在启动子处耗尽组蛋白，并且从转录单位的上游到下游富集H3.3。我们建议，沉积和遗传的积极修改H3.3在调节区保持转录活性染色质。
Histones of multicellular organisms are assembled into chromatin primarily during DNA replication. When chromatin assembly occurs at other times, the histone H3.3 variant replaces canonical H3. Here we introduce a new strategy for profiling epigenetic patterns on the basis of H3.3 replacement, using microarrays covering roughly one-third of the Drosophila melanogaster genome at 100-bp resolution. We identified patterns of H3.3 replacement over active genes and transposons. H3.3 replacement occurred prominently at sites of abundant RNA polymerase II and methylated H3 Lys4 throughout the genome and was enhanced on the dosage-compensated male X chromosome. Active genes were depleted of histones at promoters and were enriched in H3.3 from upstream to downstream of transcription units. We propose that deposition and inheritance of actively modified H3.3 in regulatory regions maintains transcriptionally active chromatin.