Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway
Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway
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DOI：
10.1016/s0092-8674(00)81725-0
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发表时间：
1998-12-23
期刊：
影响因子：
64.5
通讯作者：
Carthew, RW
中科院分区：
文献类型：
--
作者：
Kennerdell, JR;Carthew, RW
我们研究了双链RNA干扰果蝇基因功能的潜力。将dsRNA注射到胚胎中导致对测试的几个基因的有效和特异性干扰。相反，单链RNA对基因活性的干扰很弱。该方法用于确定形态发生剂Wingless的接收机制。frizzled和果蝇frizzled 2基因的干扰一起产生了胚胎模式的缺陷，模拟了无翼功能的丧失。任何一个基因单独的干扰都不会对图案形成产生影响。上位性分析表明，frizzled和果蝇frizzled 2在Wingless通路中作用于wingless的下游和zeste-white 3的上游。我们的研究结果表明，dsRNA干扰可用于分析基因功能的许多方面。
We investigated the potential of double-stranded RNA to interfere with the function of genes in Drosophila. Injection of dsRNA into embryos resulted in potent and specific interference of several genes that were tested. In contrast, single-stranded RNA weakly interfered with gene activity. The method was used to determine the reception mechanism of the morphogen Wingless. Interference of the frizzled and Drosophila frizzled 2 genes together produced defects in embryonic patterning that mimic loss of wingless function. Interference of either gene alone had no effect on patterning. Epistasis analysis indicates that frizzled and Drosophila frizzled 2 act downstream of wingless and upstream of zeste-white3 in the Wingless pathway. Our results demonstrate that dsRNA interference can be used to analyze many aspects of gene function.