ACUPUNCTURE-EVOKED RESPONSES OF SUBNUCLEUS RETICULARIS DORSALIS NEURONS IN THE RAT MEDULLA
ACUPUNCTURE-EVOKED RESPONSES OF SUBNUCLEUS RETICULARIS DORSALIS NEURONS IN THE RAT MEDULLA
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DOI：
10.1016/0306-4522(91)90088-6
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发表时间：
1991-01-01
期刊：
影响因子：
3.3
通讯作者：
LEBARS, D
中科院分区：
文献类型：
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作者：
BING, Z;VILLANUEVA, L;LEBARS, D
记录大鼠网状背侧亚核的神经元。可区分出两类神经元：一类是由激活身体任何部位的A-Delta和C-纤维驱动的完全伤害性会聚，另一类是由激活身体任何部位的A-Delta纤维或某些主要对侧区域的C-纤维驱动的部分伤害性会聚。本文研究了中医针灸“人中”、“散三里”、“长墙”、“足三里”等穴位及“足三里”旁非穴对网状背侧亚核神经元的影响。针刺“S”穴30个穴位或非穴位，强烈兴奋所有受试神经元；这些神经元以快速起效放电为主，继之以持续约30~60个S的后放电。大部分但不是全部部分伤害性感觉神经元对30个S针刺穴位或非穴位刺激有反应。当刺激被施加到身体的对侧或中线部分时，情况尤其如此。针刺作为一种激活网状背侧亚核神经元的方法，其效力随刺激部位的不同而显著不同：对侧中线和同侧区。在相同的实验条件下，诱导的活动水平与施加伤害性机械刺激所诱发的水平相似。无论刺激“足三里”穴或对侧，激活“足三里”穴与邻近非穴位网状背侧亚核神经元的能力没有差异，这表明这些神经元的激活缺乏地形图特异性。由于网状背侧亚核神经元是由伤害性输入单独或优先激活的，因此，我们得出结论，手法针刺所产生的信号通过负责伤害性信息传递的通路传递。由于针刺是一种引起广泛的节外抗伤害效应的动作，激活了背侧网状亚核神经元，从解剖学上讲，网状背侧亚核向脊髓各级的背角发送密集的投射，我们认为这一结构可能不仅参与了痛觉的信号传递，而且还通过棘网状脊髓反馈机制参与了痛觉的调制。
Recordings were made from neurons in subnucleus reticularis dorsalis of the rat. Two populations of neurons could be distinguished: those with total nociceptive convergence which were driven by activating A-delta- and C-fibers from any part of the body and those with partial nociceptive convergence which were driven by activating A delta-fibers from any part of the body or C-fibers from some, mainly contralateral, regions. The effects on subnucleus reticularis dorsalis neurons of manual acupuncture, performed by a traditional Chinese acupuncturist at the "Renzhong", "Sousanli", "Changqiang", and "Zusanli" acupoints and at a non-acupoint next to "Zusanli", were studied.Acupuncture stimulation for 30 s at the acupoints or the non-acupoint strongly excited all the total nociceptive convergence neurons tested; these neurons responded with a discharge of rapid onset which was often followed by after-discharges lasting for approximately 30-60 s. The majority but not all of the partial nociceptive convergence neurons responded to 30 s of acupuncture stimulation at the acupoints or the non-acupoint. This was especially the case when the stimulus was applied to contralateral or midline parts of the body. The potency of acupuncture as a means of activating subnucleus reticularis dorsalis neurons varied significantly with the area of the body being stimulated such that: contralateral > midline > ipsilateral areas. The levels of induced activity were of similar magnitude to those evoked by noxious mechanical stimuli applied under identical experimental conditions. No differences were found between the capacities to activate subnucleus reticularis dorsalis neurons of the "Zusanli" point and the adjacent non-acupoint, no matter whether these were stimulated ipsi- or contralaterally; this suggests a lack of topographical specificity in the activation of these neurons.Since subnucleus reticularis dorsalis neurons are activated exclusively or preferentially by noxious inputs, it is concluded that the signals elicited by manual acupuncture travel through pathways responsible for the transmission of nociceptive information. Since acupuncture, a manoeuvre which is known to elicit widespread extrasegmental antinociceptive effects, activates subnucleus reticularis dorsalis neurons which, anatomically, send dense projections to the dorsal horn at all levels of the spinal cord, we would suggest that this structure may be involved not only in signalling pain but also in modulating pain by means of spino-reticulo-spinal feed-back mechanisms.