Imaging Brain Amyloid of Alzheimer Disease in Vivo in Transgenic Mice with an Aβ Peptide Radiopharmaceutical
Imaging Brain Amyloid of Alzheimer Disease in Vivo in Transgenic Mice with an Aβ Peptide Radiopharmaceutical
复制标题
使用 Aβ 肽放射性药物对转基因小鼠体内阿尔茨海默病的脑淀粉样蛋白进行成像
DOI：
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发表时间：
2002
影响因子：
6.3
通讯作者：
W. Pardridge
中科院分区：
文献类型：
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作者：
H. Lee;Yun Zhang;Chunni Zhu;K. Duff;W. Pardridge
Aβ1–40 is a potential peptide radiopharmaceutical that could be used to image the brain Aβ amyloid of Alzheimer disease in vivo, should this peptide be made transportable through the blood–brain barrier in vivo. The blood–brain barrier transport of [125I]-Aβ1–40 in a transgenic mouse model was enabled by conjugation to the rat 8D3 monoclonal antibody to the mouse transferrin receptor. The Aβ1–40–8D3 conjugate is a bifunctional molecule that binds the blood–brain barrier TfR and undergoes transport into brain and binds the Aβ amyloid plaques of Alzheimer disease. App SW /Psen1 double-transgenic and littermate control mice were administered either unconjugated Aβ1–40 or the Aβ1–40–8D3 conjugate intravenously, and brain scans were obtained 6 hours later. Immunocytochemical analysis showed abundant Aβ immunoreactive plaques in the brains of the App SW /Psen1 transgenic mice and there was a selective retention of radioactivity in the brains of these mice at 6 hours after intravenous administration of the conjugate. In contrast, there was no selective sequestration either of the conjugate in control littermate mouse brain or of unconjugated Aβ1–40 in transgenic mouse brain. In conclusion, the results show that it is possible to image the Aβ amyloid burden in the brain in vivo with an amyloid imaging agent, provided the molecule is conjugated to a blood–brain barrier drug-targeting system.
影响因子：
7.3
作者：
Zhuang, ZP;Kung, MP;Kung, HF
通讯作者：
Kung, HF
阿尔茨海默氏病患者大脑淀粉样变性小动脉病变中免疫反应性 A4 和 γ-痕量肽的共定位。
DOI：
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发表时间：
1990
期刊：
The American journal of pathology
影响因子：
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作者：
Vinters,HV;Nishimura,GS;Secor,DL;Pardridge,WM
通讯作者：
Pardridge,WM