Viability of fibroblast-seeded ligament analogs after autogenous implantation
Viability of fibroblast-seeded ligament analogs after autogenous implantation
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DOI：
10.1002/jor.1100160404
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发表时间：
1998-07-01
影响因子：
2.8
通讯作者：
Dunn, MG
中科院分区：
文献类型：
--
作者：
Bellincampi, LD;Closkey, RF;Dunn, MG
成纤维细胞接种的胶原支架或韧带类似物可能用于膝关节前交叉韧带的重建。为了提供持久的益处，接种的细胞必须在膝关节的恶劣滑膜环境中存活植入。我们的目的是确定作为成纤维细胞来源（前交叉韧带或皮肤）、植入部位（膝关节或皮下空间）和植入后时间（1、2、4、6或8周）函数的自体成纤维细胞接种的韧带类似物的体内命运。在植入前，用荧光膜染料PKH 26-GL标记成纤维细胞。取出植入物后，立即在荧光显微镜下评估标记的接种细胞的活力。植入后，活的接种成纤维细胞保持附着于韧带类似物内的胶原纤维至少4周（皮肤成纤维细胞）或6周（前交叉韧带成纤维细胞）。在皮下间隙中始终观察到比膝关节中更多的活接种细胞。支架再吸收阻止了在8周时间段的观察。成纤维细胞接种的韧带类似物在膝关节中保持长时间的存活，因此有可能影响前交叉韧带重建后新韧带组织的形成和重塑。
Fibroblast-seeded collagen scaffolds or ligament analogs are potentially useful for reconstruction of the anterior cruciate ligament of the knee. To provide lasting benefits, the seeded cells must survive implantation within the harsh synovial environment of the knee joint. Our objective was to determine the in vivo fate of autogenous fibroblast-seeded ligament analogs as a function of fibroblast source (anterior cruciate ligament or skin), implantation site (knee joint or subcutaneous space), and time after implantation (1, 2, 4, 6, or 8 weeks). Before implantation, fibroblasts were labeled with PKH26-GL, a fluorescent membrane dye. Immediately after retrieval of the implant, the viability of the labeled seeded cells was assessed under a fluorescent microscope. Viable seeded fibroblasts remained attached to the collagen fibers within the ligament analogs for at least 4 weeks (skin fibroblasts) or 6 weeks (anterior cruciate ligament fibroblasts) after implantation. A larger number of viable seeded cells were consistently observed in the subcutaneous space than in the knee joint. Scaffold resorption prevented observation at the 8-week time period. Fibroblast-seeded ligament analogs remained viable for prolonged periods in the knee joint and therefore have the potential to influence the formation and remodeling of neoligament tissue after reconstruction of the anterior cruciate ligament.