CYTOCIDAL EFFECTS OF ESCHERICHIA-COLI HEMOLYSIN ON HUMAN T-LYMPHOCYTES
CYTOCIDAL EFFECTS OF ESCHERICHIA-COLI HEMOLYSIN ON HUMAN T-LYMPHOCYTES
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DOI：
10.1128/iai.61.5.1715-1721.1993
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发表时间：
1993-05-01
影响因子：
3.1
通讯作者：
BHAKDI, S
中科院分区：
文献类型：
--
作者：
JONAS, D;SCHULTHEIS, B;BHAKDI, S
大肠杆菌溶血素是革兰氏阴性菌产生的一大类成孔毒素的原型。除了已知的对粒细胞、单核细胞、内皮细胞和肾上皮细胞的细胞毒活性外，我们现在证明这种毒素能杀死人T淋巴细胞。基于不同和独立方法的证据表明，淋巴细胞杀灭活性是由于跨膜孔的形成。此外，植物血凝素预刺激的细胞对低剂量大肠杆菌溶血素的DNA片段反应类似于在经历程序性细胞死亡的细胞中观察到的。动力学方面的考虑使我们得出结论，DNA降解可能只是一种附带现象。杀死T细胞是大肠杆菌溶血素直接破坏宿主防御的另一种方式。
Escherichia coli hemolysin is the prototype of a large family of pore-forming toxins produced by gram-negative organisms. Besides its known cytotoxic activities against granulocytes, monocytes, endothelial cells, and renal epithelial cells, we now demonstrate that the toxin potently kills human T lymphocytes. Evidence based on different and independent approaches indicates that lymphocidal activity is due to formation of transmembrane pores. Additionally, cells prestimulated with phytohemagglutinin respond to low doses of E. coli hemolysin with DNA fragmentation similar to that observed in cells undergoing programmed cell death. Kinetic considerations lead us to conclude that DNA degradation may, however, represent an epiphenomenon. Killing of T cells is another means through which E. coli hemolysin could directly impair host defense.