Ribonucleic acid polymerase mutation affecting glutamate synthase activity in and sporulation of Bacillus subtilis
Ribonucleic acid polymerase mutation affecting glutamate synthase activity in and sporulation of Bacillus subtilis
复制标题
影响枯草芽孢杆菌谷氨酸合酶活性和孢子形成的核糖核酸聚合酶突变
DOI：
--
复制
发表时间：
1979
影响因子：
3.2
通讯作者：
S. Takayanagi
中科院分区：
文献类型：
--
作者：
J. Ryu;S. Takayanagi
研究了枯草芽孢杆菌168菌株的15个利福平抗性（Rifr）突变体的孢子形成。作为Rifr突变的多效性效应，谷氨酸合酶活性在这些突变体中丧失。15个突变体中有12个形成了与亲本一样多的孢子，但其余3个形成的孢子明显较少（1%）。进一步表征的后者突变体之一（RF301）在其孢子形成过程中在第0阶段被阻断。因此，可以得出结论，核糖核酸聚合酶的某种修饰可能会特异性地影响谷氨酸合成酶基因的表达，并在初始阶段的孢子形成过程。
Spore formation of 15 rifampin-resistant (Rifr) mutants of Bacillus subtilis strain 168 was examined. As a pleiotropic effect of a Rifr mutation, glutamate synthase activity was lost in these mutants. Twelve of the 15 mutants examined formed as many spores as the parent, but the remaining 3 formed significantly fewer (1%) spores. One of the latter mutants characterized further (RF301) was blocked in its sporulation process at stage 0. Thus, it was concluded that a certain modification of ribonucleic acid polymerase may affect specifically the gene expression of glutamate synthase and also the sporulation process at the initial stage.